Structure of an acidic phospholipase A(2) from the venom of Deinagkistrodon acutus in a new crystal form
文献类型：期刊论文
| 作者 | Gu, LC; Zhang, HL; Song, SY; Zhou, YC; Lin, ZJ |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2002 |
| 卷号 | 34期号:3页码:266-272 |
| 关键词 | phospholipase A(2) X-ray crystallography three-dimensional structure hemolysis interfacial catalysis |
| 通讯作者 | , |
| 英文摘要 | The three-dimensional structure of an acidic phospholipase A purified from the venom of Deinagkistrodon acutus ( Agkistrodon acutus) was determined in a new crystal form by molecular replacement at 0.28 nm resolution with a crystallographic R factor of 21.9% ( R-free = 25.7%) and reasonable stereochemistry. Being similar to the previous reported crystal form, a significant conformational adaptation of segment 14-23 at the dimer interface was observed. This segment was related to the "interface recognition site" (IRS). It was found that a positively charged residue at position 34 seems to be a common feature for most of hemolytic PLA(2)s belonging to group II. Structural comparison between the two crystal forms showed that NaCl had significant effects on the crystal packing, thus leading to dramatic changes of the unit cell parameters. In the new crystal form, MPD (2-methyl-2, 4-pentanediol) molecules exist in the hydrophobic channel of the enzyme. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | AGKISTRODON-HALYS-PALLAS ; NAJA-NIGRICOLLIS ; CHEMICAL MODIFICATION ; VIPERA-RUSSELLI ; SEQUENCE ; CLONING ; INTERFACES ; PURIFICATION ; RESOLUTION ; BLOMHOFFII |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000175697900002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2462]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Gu, LC,Zhang, HL,Song, SY,et al. Structure of an acidic phospholipase A(2) from the venom of Deinagkistrodon acutus in a new crystal form[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(3):266-272. |
| APA | Gu, LC,Zhang, HL,Song, SY,Zhou, YC,&Lin, ZJ.(2002).Structure of an acidic phospholipase A(2) from the venom of Deinagkistrodon acutus in a new crystal form.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(3),266-272. |
| MLA | Gu, LC,et al."Structure of an acidic phospholipase A(2) from the venom of Deinagkistrodon acutus in a new crystal form".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.3(2002):266-272. |
入库方式： OAI收割
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