Purification and characterization of L-amino acid oxidase from Agkistrodon halys Pallas venom
文献类型：期刊论文
| 作者 | Liu, JW; Chai, MQ; Du, XY; Song, JG; Zhou, YC |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2002 |
| 卷号 | 34期号:3页码:305-310 |
| 关键词 | L-amino acid oxidase ( LAO) Agkistrodon hulys Pallas platelet aggregation antibacterial effect apoptosis |
| 通讯作者 | , |
| 英文摘要 | L-amino acid oxidase (LAO, EC 1. 4. 3. 2) is widely found in snake venoms and is thought to Contribute to the toxicity in envenoming. By using of Sephadex G-150, DEAE-Sepharose CL-6B and FPLC Superose 12 chromatography, a protein with L-amino acid oxidase activity was purified and characterized from Agkistrodon haly Pallas venom. Its molecular mass was 57 kD as determined by SDS-PAGE analysis under both reducing and non-reducing conditions, and its pI was about 4.9. The protein catalysed the stereospecific oxidative deamination of L-amino acid substrate. It inhibited the platelet aggregation induced by ADP and collagen dose-dependently, even at low concentrations of 0.2 mumol/L and 0.08 mumol/L, respectively. The LAO had antibacterial effect to E. coli K12D31, and the effective concentration was as low as 0.03 g/L. Furthermore, the LAO showed cytotoxicity in crystal violet assay and apoptosis-inducing acitvity in the A549 culls. After 24 h treatment with 5 mg/L LAO, the typical DNA fragmentation pattern of apoptotic culls was observed by using of agrose gel electrophoresis. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | APOPTOSIS-INDUCING FACTOR ; COBRA OPHIOPHAGUS HANNAH ; SNAKE-VENOM ; PLATELET-AGGREGATION ; PROTEIN |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000175697900008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2464]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, JW,Chai, MQ,Du, XY,et al. Purification and characterization of L-amino acid oxidase from Agkistrodon halys Pallas venom[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(3):305-310. |
| APA | Liu, JW,Chai, MQ,Du, XY,Song, JG,&Zhou, YC.(2002).Purification and characterization of L-amino acid oxidase from Agkistrodon halys Pallas venom.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(3),305-310. |
| MLA | Liu, JW,et al."Purification and characterization of L-amino acid oxidase from Agkistrodon halys Pallas venom".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.3(2002):305-310. |
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