Cloning and expression of gynecophoral canal protein gene of Schistosoma japonicum (Chinese strain)
文献类型：期刊论文
| 作者 | Jin, YM; Lin, JJ; Feng, XG; Zhang, L; Wu, XF; Zhou, YC; Cai, YM |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2002 |
| 卷号 | 34期号:3页码:311-317 |
| 关键词 | Schistosoma japonicum gynecophoral canal protein gene cloning gene expression |
| 通讯作者 | , |
| 英文摘要 | A 1949 bp cDNA fragment was amplified by RT-PCR from adult Schistosoma japonicum (Chinese strain) mRNA with 3 pair of primers that were designed according to published SmGCP gene encoding gynecophoral canal protein of Schistosoma mansoni and SjGCP1 gene encoding the conservative region of gynecophoral canal protein of Schistosoma japonicum. Sequence analysis indicated that this fragment, named SjGCP, with 85% identity to SmGCP, contained a complete open reading frame (ORF) of gynecophoral canal protein gene of Schistosoma japonicum (Chinese strain). The amino acid sequence shared 83.7% identity with gynecophoral canal protein of Schistosoma mansoni. This fragment was cloned into the expression vector pET28c(+) and subsequently expressed in Escherichia coli. SDS-PAGE revealed that the molecular weight of this expressed product was 80 kD. Western blotting showed that the recombinant protein reacted well with the rabbit serum immunized with Sj worm antigen, indicating that this expressed product had good antigenicity. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | ESCHERICHIA-COLI ; WORMS |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000175697900009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2465]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Jin, YM,Lin, JJ,Feng, XG,et al. Cloning and expression of gynecophoral canal protein gene of Schistosoma japonicum (Chinese strain)[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(3):311-317. |
| APA | Jin, YM.,Lin, JJ.,Feng, XG.,Zhang, L.,Wu, XF.,...&Cai, YM.(2002).Cloning and expression of gynecophoral canal protein gene of Schistosoma japonicum (Chinese strain).ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(3),311-317. |
| MLA | Jin, YM,et al."Cloning and expression of gynecophoral canal protein gene of Schistosoma japonicum (Chinese strain)".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.3(2002):311-317. |
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