The hemolytic site of the basic phospholipase A(2) from Agkistrodon halys Pallas
文献类型：期刊论文
| 作者 | Jiao, HM; Jin, Q; Zhao, JJ; Feng, B; Wu, XF; Zhou, YC |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2002 |
| 卷号 | 34期号:3页码:383-387 |
| 关键词 | Agkistrodon halys Pallas basic phospholipase A(2) hemolysis |
| 通讯作者 | , |
| 英文摘要 | The gene of the basic phospholipase A(2) from Agkistrodon halys Pallas (BPLA(2))was mutated site-directedly by polymerase chain reaction (PCR) and the residue Arg(34) of the encloding protein was substituted by Glu and Gln, respectively. The mutant gene has been cloned into the expression vector pBLMVL2 and has been expressed in E. coli RR1 effectively, The protein was produced as insoluble inclusion bodies. After partial purification, the inclusion bodies were denatured and renatured into active form, and the renatured recombinant protein was purified by gel-filtration. The expression product has the same enzymatic activity as the denatured-refolded BPLA(2) and its hemolytic activity dropped distinctly, which suggest that the basic residue Arg(34) of BPLA(2) is a crucial amino acid residue during the process of hemolysis. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | VENOM PHOSPHOLIPASE-A2 ; ESCHERICHIA-COLI ; SNAKE-VENOMS ; EXPRESSION ; GENE ; NEUROTOXIN ; CLONING |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000175697900023 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2467]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Jiao, HM,Jin, Q,Zhao, JJ,et al. The hemolytic site of the basic phospholipase A(2) from Agkistrodon halys Pallas[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(3):383-387. |
| APA | Jiao, HM,Jin, Q,Zhao, JJ,Feng, B,Wu, XF,&Zhou, YC.(2002).The hemolytic site of the basic phospholipase A(2) from Agkistrodon halys Pallas.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(3),383-387. |
| MLA | Jiao, HM,et al."The hemolytic site of the basic phospholipase A(2) from Agkistrodon halys Pallas".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.3(2002):383-387. |
入库方式： OAI收割
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