Secretory phospholipase A(2) induces apoptosis via a mechanism involving ceramide generation
文献类型：期刊论文
| 作者 | Zhao, S; Du, XY; Chai, MQ; Chen, JS; Zhou, YC; Song, JG |
| 刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
 |
| 出版日期 | 2002 |
| 卷号 | 1581期号:3页码:75-88 |
| 关键词 | apoptosis ceramide phospholipase D secretory phospholipase A(2) sphingomyelinase sphingomyelin |
| 通讯作者 | Song, JG (reprint author), Chinese Acad Sci, Shanghai Inst Biochem, Box 25,320 Yueyang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Secretory phospholipase A(2) (sPLA(2)) plays important roles in cellular signaling and various biological events. In this study, we examined the biological effects and the potential signaling mechanism of purified sPLA(2) in MV 1 Lu cells. Three types of snake venom sPLA(2) were purified and their enzymatic activities were characterized by using various lipid substrates prepared from [H-3]-myristate-labeled cells and by determining their effects on the induction of arachidonic acid (AA) release. The purified sPLA, induced apoptosis in Mv 1 Lu cells in a dose- and time-dependent manner, and was associated with a rapid increase in the intracellular ceramide level. Similar apoptotic effects were observed in Mv I Lit cells treated with exogenous ceramide analog, C-2- and C-8-ceramide. Moreover, treatment of cells with sphingomyelinase (SMase), which reduced the intracellular SM level, enhanced the apoptotic response to sPLA(2)s. sPLA(2)s also displayed an inhibitory effect on bradykinin-induced phospholipase D (PLD) activity, which can be imitated by exogenous ceramide. Our data indicate that sPLA(2) induces cell apoptosis via a mechanism involving increased ceramide generation. (C) 2002 Elsevier Science B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 关键词[WOS] | DAUNORUBICIN-INDUCED APOPTOSIS ; PLATELET-ACTIVATING-FACTOR ; NECROSIS-FACTOR-ALPHA ; SPHINGOMYELIN HYDROLYSIS ; SIGNAL-TRANSDUCTION ; PHOSPHATIDIC-ACID ; ARACHIDONIC-ACID ; CELLS ; SPHINGOLIPIDS ; PHOSPHATIDYLCHOLINE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000176018200002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2468]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhao, S,Du, XY,Chai, MQ,et al. Secretory phospholipase A(2) induces apoptosis via a mechanism involving ceramide generation[J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS,2002,1581(3):75-88. |
| APA | Zhao, S,Du, XY,Chai, MQ,Chen, JS,Zhou, YC,&Song, JG.(2002).Secretory phospholipase A(2) induces apoptosis via a mechanism involving ceramide generation.BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS,1581(3),75-88. |
| MLA | Zhao, S,et al."Secretory phospholipase A(2) induces apoptosis via a mechanism involving ceramide generation".BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS 1581.3(2002):75-88. |
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