In vitro interaction of eukaryotic elongation factor 2 with synthetic oligoribonucleotide that mimics GTPase domain of rat 28S ribosomal RNA
文献类型：期刊论文
| 作者 | He, WJ; Tang, SA; Liu, WY |
| 刊名 | INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
 |
| 出版日期 | 2002 |
| 卷号 | 34期号:3页码:263-268 |
| 关键词 | elongation factor 2 (eEF2) GTPase domain RNA GTP-binding protein ADP-ribosylation interaction |
| 通讯作者 | Liu, WY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Eukaryotic elongation factor 2 (eEF2) catalyzed the translocation of peptidyl-tRNA from the ribosomal A site to the P site. In this paper, the interaction between eEF2 and GTD RNA, a synthetic oligoribonucleotide that mimicked the GTPase domain of rat 28S ribosomal RNA, was Studied in vitro, The purified eEF2 could bind to GTD RNA, forming a stable complex. Transfer RNA competed with GTD RNA in binding to eEF2, whereas poly(A), poly(U) and poly(I,C) did not interfere with the interaction between eEF2 and GTD RNA, demonstrating that the tertiary structure of RNA might be necessary for the recognition of and binding to eEF2. The complex formation of eEF2 with GTD RNA was inhibited by SRD RNA, a synthetic oligoribonucleotide mimic of Sarcin/Ricin domain RNA of rat 28S RNA. Similarly, GTD RNA inhibited the interaction between eEF2 and SRD RNA. This fact implies that these small oligoribonucleotides probably share similar recognition or binding identity elements in their tertiary structures. In addition, the binding of eEF2 to GTD RNA could be obviously weakened by the ADP-ribosylation of eEF2 with diphtheria toxin. These results indicate that cEF2 behaves differently from prokaryotic EF-G in binding to ribosomal RNA. (C) 2002 Elsevier Science Ltd. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | FACTOR EF-G ; FACTOR-II ; ADP-RIBOSYLATION ; ANGSTROM-RESOLUTION ; PROTEIN-SYNTHESIS ; DIPHTHERIA-TOXIN ; BINDING ; POLYRIBOSOMES ; AUTOANTIBODY ; SUBUNIT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000174113400006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2471]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | He, WJ,Tang, SA,Liu, WY. In vitro interaction of eukaryotic elongation factor 2 with synthetic oligoribonucleotide that mimics GTPase domain of rat 28S ribosomal RNA[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2002,34(3):263-268. |
| APA | He, WJ,Tang, SA,&Liu, WY.(2002).In vitro interaction of eukaryotic elongation factor 2 with synthetic oligoribonucleotide that mimics GTPase domain of rat 28S ribosomal RNA.INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,34(3),263-268. |
| MLA | He, WJ,et al."In vitro interaction of eukaryotic elongation factor 2 with synthetic oligoribonucleotide that mimics GTPase domain of rat 28S ribosomal RNA".INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 34.3(2002):263-268. |
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