Argipressin(4-8) upregulate CTP: Phosphocholine cytidylyltransferase in rat hippocampal neurons
文献类型：期刊论文
| 作者 | Xu, KY; Xiong, Y; Du, YC |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2002 |
| 卷号 | 34期号:4页码:418-422 |
| 关键词 | argipressin CTP phosphocholine cytidylyltransferase hippocampus phosphatidylcholine actinomycin D |
| 通讯作者 | , |
| 英文摘要 | In order to study the effect of argipressin(4-8) (AVP(4-8)) on the mRNA level and activity of cytidine triphosphate: phosphocholine cytidylyltransferase (CCT) in rat hippocampal neurons, and elucidate its possible mechanism. Rat hippocampal neurons treated with AVP(4-8) or actinomycin D were incubated with different time periods. The mRNA level of CCT was detected using RT-PCR plus Southern blot, CCT activity was determined by measuring the rate of incorporation of [C-14]- phosphocholine into cytidine diphosphate-choline (CDP-choline). It was found that AVP(4-8) could upregulate the CCT mRNA in rat hippocampal neurons. ZDC(C)PR, the antagonist of AVP(4-8), could greatly inhibit this upregulation. Using actinomycin D to inhibite the eucaryotic transcription, it was found that the halflife of CCT mRNA could be prolonged by coincubation with AVP(4-8). Meanwhile, AVP(4-8) could also increase CCT activity in rat hippocampal neurons. These results demonstrated that AVP(4-8) upregulated CCT mRNA level and its activity through stabilizing the CCT mRNA in rat hippocampal neurons. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | BRAIN ; CELLS ; PHOSPHORYLATION ; FACILITATION ; AVP(4-8) ; ANALOGS ; CLONING |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000177017300006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2479]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xu, KY,Xiong, Y,Du, YC. Argipressin(4-8) upregulate CTP: Phosphocholine cytidylyltransferase in rat hippocampal neurons[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(4):418-422. |
| APA | Xu, KY,Xiong, Y,&Du, YC.(2002).Argipressin(4-8) upregulate CTP: Phosphocholine cytidylyltransferase in rat hippocampal neurons.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(4),418-422. |
| MLA | Xu, KY,et al."Argipressin(4-8) upregulate CTP: Phosphocholine cytidylyltransferase in rat hippocampal neurons".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.4(2002):418-422. |
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