Sequence analysis of F gene of SF02 isolate of goose paramyxovirus and its identification by multiplex RT-PCR
文献类型：期刊论文
| 作者 | Zou, J; Shan, SH; Yao, LT; Gong, ZX |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2002 |
| 卷号 | 34期号:4页码:439-444 |
| 关键词 | GPV NDV F gene sequence analysis multiplex RT-PCR |
| 通讯作者 | , |
| 英文摘要 | The complete F gene of SF02 of goose paramyxovirus (GPV) has been cloned and analyzed. The sequence analysis demonstrated that the F gene of SF02 contains 1 662 nt and encodes 553 amino acids, and its cleavage activation site of F gene has the same deduced amino acid sequence, R-112-R-Q-K-R-F-117, as the velogenic (highly pathogenic) strain of newcastle disease virus. The latter correlated with the virulence of the isolate in biological assays. The F gene of SR2 isolate with the domestic standard velogenic strain NDV, F48E9, shared 86.5% homology in nucleotide and 90.8% homology in amino acid sequences. The SF02 isolate is closer to some NDV strains prevalent in Taiwan and West-European countries in recent years. Based on the F gene sequence a multiplex RT-PCR method has been developed. It could be used for the discrimination of GPV from NDV. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | WESTERN-EUROPE ; GENOTYPE-VII ; OUTBREAKS |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000177017300009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2482]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zou, J,Shan, SH,Yao, LT,et al. Sequence analysis of F gene of SF02 isolate of goose paramyxovirus and its identification by multiplex RT-PCR[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(4):439-444. |
| APA | Zou, J,Shan, SH,Yao, LT,&Gong, ZX.(2002).Sequence analysis of F gene of SF02 isolate of goose paramyxovirus and its identification by multiplex RT-PCR.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(4),439-444. |
| MLA | Zou, J,et al."Sequence analysis of F gene of SF02 isolate of goose paramyxovirus and its identification by multiplex RT-PCR".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.4(2002):439-444. |
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