DNA immunization with recombinant HCV E2 expression plasmids
文献类型：期刊论文
| 作者 | Zhu, J; Wang, CL; Zhu, LX; Kong, YY; Wang, Y; Li, GD |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2002 |
| 卷号 | 34期号:4页码:445-451 |
| 关键词 | HCV E2 HBV preS1 promoter signal sequence DNA vaccine |
| 通讯作者 | , |
| 英文摘要 | DNA-based immunization, the delivery of plasmid DNA by direct inoculation, is a newly developed method of vaccination. Besides of many advantages, DNA immunization was demonstrated to generate weaker antibody and CTL responses than did protein and live attenuated vaccines, respectively. To circumvent this shortage, several methods were tested such as using different vector, changing injection mode, and co-expressing cytokines, etc. The studies showed that many factors could greatly affect the immune responses to DNA vaccine. The aim of this study is to compare different vectors for the presentation of the HCV E2 to generate immune responses by using DNA-based immunization. Four expression plasmids, with different promoter types and with or without signal sequences were constructed, which encode C-terminally truncated E2 (384-660) with HBV preS1(21-47) tag fused to its N-termint. Transient expression in HeLa cells showed that only recombinant plasmids with signal sequence could be expressed and properly processed, and the product could be secreted into medium. Protein expression level is slightly higher in plasmids with CW promoter than with EF1alpha promoter. After immunization of C57BL/6 mice, all the recombinant constructs could elicit both anti-preS1 and IV promoter and signal sequence could induce anti-E2 antibodies. But only pCWSec-S1E2t660 with both CMV high-level and long-lasting antibody, showing that it is a good vaccine candidate. Possible reasons for the different immune responses to these constructs were discussed. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | HEPATITIS-C VIRUS ; B SURFACE-ANTIGEN ; IMMUNE-RESPONSES ; VACCINATION ; PROTEINS ; GENE ; INDUCTION ; MICE ; PURIFICATION ; INOCULATION |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000177017300010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2483]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhu, J,Wang, CL,Zhu, LX,et al. DNA immunization with recombinant HCV E2 expression plasmids[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(4):445-451. |
| APA | Zhu, J,Wang, CL,Zhu, LX,Kong, YY,Wang, Y,&Li, GD.(2002).DNA immunization with recombinant HCV E2 expression plasmids.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(4),445-451. |
| MLA | Zhu, J,et al."DNA immunization with recombinant HCV E2 expression plasmids".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.4(2002):445-451. |
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