PEG and DBBF modified porcine hemoglobin and their oxygen-carrying capacity
文献类型：期刊论文
| 作者 | Hong, M; Cai, J; Meng, WF; Li, SY; Yuan, ZY |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2002 |
| 卷号 | 34期号:4页码:452-456 |
| 关键词 | porcine hemoglobin PEG DBBF modification blood substitute |
| 通讯作者 | , |
| 英文摘要 | Modifications of proteins with polyethylene glycol (PEG) have been proven to enlarge molecule size of proteins, to prolong their retention time in the circulation as well as blunt immune or allergic reactions. Hemoglobin cross-linked with small molecular modifiers turns out to be more stable and to have better oxygen carrying capacity. In the present study, four derivatives of PEG with different activation groups, and several PEGs with different molecular weights were covalently bound to porcine hemoglobin (pHb). PEG-pHbs exhibited a variety of differences in their properties depending on the molecular weights of the used PEGs, the amounts of bound PEGs and the presence or absence of allosteric cofactors. The optimal modification conditions for his (3, 5-dibromosalicyl) fumarate (DBBF) as well as the physical features and oxygen carrying capacity of DBBF-modified pHb were evaluated. Furthermore, both PEG and DBBF were used simultaneously to modify pHb. The results indicate that the pHbs modified with PEG and DBBF have more stable tetrameric conformations with a molecular weight of 107 kD. Their oxygen half-saturation pressure (P-50) is around 3.33 kPa which approximates the physiological P50 of human red blood cells. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | POLYETHYLENE-GLYCOL |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000177017300011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2484]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hong, M,Cai, J,Meng, WF,et al. PEG and DBBF modified porcine hemoglobin and their oxygen-carrying capacity[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(4):452-456. |
| APA | Hong, M,Cai, J,Meng, WF,Li, SY,&Yuan, ZY.(2002).PEG and DBBF modified porcine hemoglobin and their oxygen-carrying capacity.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(4),452-456. |
| MLA | Hong, M,et al."PEG and DBBF modified porcine hemoglobin and their oxygen-carrying capacity".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.4(2002):452-456. |
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