Localization of CCT beta in rat brain and overexpression in insect cells
文献类型：期刊论文
| 作者 | Xu, KY; Xiong, Y; Xie, ZQ; Bian, W; Jing, NH; Du, YC |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2002 |
| 卷号 | 23期号:4页码:349-354 |
| 关键词 | CTP phosphocholine cytidylyltransferase argipressin in situ hybridization hippocampus hexadecylphosphocholine baculovirus |
| 通讯作者 | Du, YC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | AIM: To study the localization of CTP: phosphocholine cytidylyltransferase beta isoform (CCTbeta) in rat brain, its expression in insect cells and enzymatic properties. METHODS: Using digoxigenin-labeled CCTbeta probes, in situ hybridization was carried out in rat brain wax sections. CCTbeta was overexpressed in Trichoplusia Ni (Tn) cells using baculovirus expression system. CTP: phosphocholine cytidylyltransferase assay (CT assay) and [H-3] metabolic labeling experiment were used to study its activity, properties, and the effect on phosphatidylcholine (PC) synthesis. RESULTS: (1) CCTbeta was abundant in CA1, CA2, CA4, and dentate gyrus (DG) region of hippocampus. (2) The content of CCTbeta in transfected Tn cells was over 1 x 10(4) times of that in rat brain, and CCTbeta increased the PC synthesis of Th cells, (3) Hexadecylphosphocholine as well as some ions like Zn2+ and PO43- could inhibit the activity of CCTbeta, dCTP was another adaptive substrate of CCTbeta besides CTP. CONCLUSION: CCTbeta showed a similar localization in rat brain with the memory enhancing peptide argipressin (4-8). |
| 学科主题 | Chemistry; Pharmacology & Pharmacy |
| 类目[WOS] | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 关键词[WOS] | CTP-PHOSPHOCHOLINE CYTIDYLYLTRANSFERASE ; CHOLINE ; CLONING ; MEMORY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000174791500013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2489]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xu, KY,Xiong, Y,Xie, ZQ,et al. Localization of CCT beta in rat brain and overexpression in insect cells[J]. ACTA PHARMACOLOGICA SINICA,2002,23(4):349-354. |
| APA | Xu, KY,Xiong, Y,Xie, ZQ,Bian, W,Jing, NH,&Du, YC.(2002).Localization of CCT beta in rat brain and overexpression in insect cells.ACTA PHARMACOLOGICA SINICA,23(4),349-354. |
| MLA | Xu, KY,et al."Localization of CCT beta in rat brain and overexpression in insect cells".ACTA PHARMACOLOGICA SINICA 23.4(2002):349-354. |
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