Bioactivities of ricin retained and its immunoreactivity to anti-ricin polyclonal antibodies alleviated through pegylation
文献类型：期刊论文
| 作者 | Hu, RG; Zhai, QW; He, WJ; Mei, L; Liu, WY |
| 刊名 | INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
 |
| 出版日期 | 2002 |
| 卷号 | 34期号:4页码:396-402 |
| 关键词 | cytotoxicity mPEG PEG pegylation immunotoxin RNA N-glycosidase |
| 通讯作者 | Liu, WY (reprint author), Chinese Acad Sci, Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 20031, Peoples R China., |
| 英文摘要 | Ricin has long been employed to construct immunotoxins, whose efficacy was often undermined by immunogenicity. Pegylation (modification of proteins with polyethylene glycol, PEG) was one of those recently developed approaches to circumvent immunogenicity of legions of drugs. Herein, pegylation of ricin was found to have barely changed the RNA N-glycosidase activity and protein synthesis inhibiting activity of ricin, but remarkably altered the cytotoxicity of ricin on hepatoma cell line (BEL7404) or the immunoreactivity with polyclonal anti-ricin antibodies. This result suggested that the attached PEG or monomethyloxyl polyethylene glycol (mPEG) groups did not hinder ricin from hydrolyzing ribosomal RNA, but indeed covered some areas on the surface of ricin molecule, including those involved in. the interaction with cellular receptors and epitopes recognized by polyclonal antibodies. Pegylation, masking certain epitopes of ricin, might contribute to alleviate the immunogenicity of the toxin. Approach in this work, if applied to thereby constructed immunotoxins, would help improve the prospective efficacy of these toxins. (C) 2002 Elsevier Science Ltd. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | NECROSIS-FACTOR-ALPHA ; POLYETHYLENE-GLYCOL ; CHEMICAL MODIFICATION ; ANTITUMOR POTENCY ; MOLECULAR DESIGN ; IN-VIVO ; PROTEINS ; SITE ; IMMUNOGENICITY ; TOXICITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000174523100011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2490]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hu, RG,Zhai, QW,He, WJ,et al. Bioactivities of ricin retained and its immunoreactivity to anti-ricin polyclonal antibodies alleviated through pegylation[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2002,34(4):396-402. |
| APA | Hu, RG,Zhai, QW,He, WJ,Mei, L,&Liu, WY.(2002).Bioactivities of ricin retained and its immunoreactivity to anti-ricin polyclonal antibodies alleviated through pegylation.INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,34(4),396-402. |
| MLA | Hu, RG,et al."Bioactivities of ricin retained and its immunoreactivity to anti-ricin polyclonal antibodies alleviated through pegylation".INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 34.4(2002):396-402. |
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