Expression of TnIAP in Trichoplusia ni cells and its biological activity
文献类型：期刊论文
| 作者 | Liao, WT; Yang, Y; Wu, XF |
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2002 |
| 卷号 | 29期号:5页码:741-744 |
| 关键词 | TnIAP expression apoptosis |
| 通讯作者 | , |
| 英文摘要 | TnIAP, which can inhibit apoptosis and was cloned from Tri-5B1-4 cells, was highly expressed in Tn-5B1-4 cells. However, SDS-PAGE and Western blot analysis indicated that only small part of recombinant protein was soluble, which was different from the record that most recombinant protein expressed in insect cells were soluble. Activity assays demonstrated that recombinant soluble TnIAP could inhibit the cleavage of Ac-LEHD-AFC by caspase-9 and the activation of HEK293 cytosolic extracts by caspase-9 from cleaving Ac-DEVD-AFC. This result gave the further evidence that the molecular mechanism of apoptosis is evolutionarily conserved through insects to mammals. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | CASPASE ACTIVATION ; APOPTOSIS ; IAP |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000178956400016 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2497]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liao, WT,Yang, Y,Wu, XF. Expression of TnIAP in Trichoplusia ni cells and its biological activity[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2002,29(5):741-744. |
| APA | Liao, WT,Yang, Y,&Wu, XF.(2002).Expression of TnIAP in Trichoplusia ni cells and its biological activity.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,29(5),741-744. |
| MLA | Liao, WT,et al."Expression of TnIAP in Trichoplusia ni cells and its biological activity".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 29.5(2002):741-744. |
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