Cloning and high-level expression of scorpion toxin BmKITa1 in Escherichia coli and insect cells
文献类型：期刊论文
| 作者 | Liu, Z; Yang, GZ; Chi, CW; Wu, XF |
| 刊名 | PROTEIN AND PEPTIDE LETTERS
 |
| 出版日期 | 2002 |
| 卷号 | 9期号:5页码:419-426 |
| 关键词 | BmKITa1 E. coli baculovirus expression system Tn-5B1-4 cell |
| 通讯作者 | , |
| 英文摘要 | BmK ITal cDNA was cloned and highly expressed in E. coli and insect cell. SDS-PAGE and western blot analysis revealed that subunit molecular weight of expression products is about 40 kDa and 10 kDa respectively. The expression product purified by a Ni2+-IDA-sepharose 6B column was toxic for insect, which indicated that it was biologically activity. Furthermore, Quantitative estimation show that the biological activity of recombinant BmK ITal from Tn cells was more powerful than from K coli. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | BUTHUS-MARTENSI KARSCH ; SELECTIVE NEUROTOXIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000177915300007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2498]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, Z,Yang, GZ,Chi, CW,et al. Cloning and high-level expression of scorpion toxin BmKITa1 in Escherichia coli and insect cells[J]. PROTEIN AND PEPTIDE LETTERS,2002,9(5):419-426. |
| APA | Liu, Z,Yang, GZ,Chi, CW,&Wu, XF.(2002).Cloning and high-level expression of scorpion toxin BmKITa1 in Escherichia coli and insect cells.PROTEIN AND PEPTIDE LETTERS,9(5),419-426. |
| MLA | Liu, Z,et al."Cloning and high-level expression of scorpion toxin BmKITa1 in Escherichia coli and insect cells".PROTEIN AND PEPTIDE LETTERS 9.5(2002):419-426. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。