Redox properties and conformational changes of DsbA protein from Escherichia coli periplasm
文献类型：期刊论文
| 作者 | Li, Q; Hu, HY |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2002 |
| 卷号 | 34期号:5页码:583-588 |
| 关键词 | DsbA protein redox property conformational change site-directed mutagenesis Trp-analog labeling |
| 通讯作者 | , |
| 英文摘要 | DsbA protein, a disulfide bond enzyme in Escherichia coli periplasm, catalyzes mainly disulfide bond formation of proteins. Site-directed mutagenesis combined with Trp-analog labeling technique was used to investigate the redox properties and conformational changes of DsbA. The results show that: (1) DsbA, as an oxidase, can catalyze disulfide bond formation efficiently. The reduced form is more stable than the oxidized form, suggesting that the strong oxidizing force of DsbA comes from the: tense conformation of the oxidized form. (2) The alteration of local environment around Trp(76) between redox forms is responsible for the special fluorescence phenomenon of DsbA. (3) The result from F-19-NMR study provides further evidence that the local conformation of Trp(76) is dramatically affected during the transformation of redox forms, but that of Trp(126) remains unaffected. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | BOND FORMATION INVIVO ; EFFICIENT CATALYSIS ; DISULFIDE-ISOMERASE ; THIOREDOXIN ; DOMAIN |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000178180200009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2505]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, Q,Hu, HY. Redox properties and conformational changes of DsbA protein from Escherichia coli periplasm[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(5):583-588. |
| APA | Li, Q,&Hu, HY.(2002).Redox properties and conformational changes of DsbA protein from Escherichia coli periplasm.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(5),583-588. |
| MLA | Li, Q,et al."Redox properties and conformational changes of DsbA protein from Escherichia coli periplasm".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.5(2002):583-588. |
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