Identification of a snoRNA47 gene cluster in Oryza sativa
文献类型：期刊论文
| 作者 | Jiang, G; Li, W; Jin, YX; Wang, DB |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2002 |
| 卷号 | 34期号:5页码:667-670 |
| 关键词 | box C/D snoRNA OSsnR47 gene cluster rRNA methylation |
| 通讯作者 | , |
| 英文摘要 | Small nucleolar RNAs (snoRNAs) are required for ribose 2-O-methylation of eukaryotic ribosomal RNA. By researching in international rice genome databases, a snoRNA gene cluster, consisting of three box C/D snoRNA gene candidates, was found on chromosome 6. All of snoRNA coding sequences in this cluster exhibited the characteristic structure of box C/D antisense snoRNA. They shared conserved box C and box D motifs, a stable terminal stem formed by a 4-6 nt-long inverted repetition sequence located upstream of box C and downstream of box D. All candidates had a 12 nt-long sequence complementary to the region from 621 nt to 632 nt in rice 18 S rRNA (nt numbering according to GenBank accession No. X00755), and might mediate the ribose 2 -O-methylation of A(623) in rice 18 S rRNA. Comparison of these sequences showed that these three rice snoRNAs were homologue of yeast snR47. These three rice snoRNAs were named as OSsnR47.1, OSsnR47.2 and OSsnR47.3, respectively. Primer extension assay showed that these: three snoRNAs were transcribed in vivo and determined the 5 ends of each snoRNA. RT-PCR detected transcripts containing linked snoRNA47s and this suggested that the snoRNAs encoded in the cluster might be transcribed as a polycistronic transcript under the control of a single upstream promoter. The gene sequences encoding these three snoRNAs had been deposited in GenBank under accession number of AF453504, AF453503 and AF453502. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | SMALL NUCLEOLAR RNAS ; ARABIDOPSIS-THALIANA ; RIBOSOMAL-RNA ; METHYLATION |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000178180200023 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2507]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Jiang, G,Li, W,Jin, YX,et al. Identification of a snoRNA47 gene cluster in Oryza sativa[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(5):667-670. |
| APA | Jiang, G,Li, W,Jin, YX,&Wang, DB.(2002).Identification of a snoRNA47 gene cluster in Oryza sativa.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(5),667-670. |
| MLA | Jiang, G,et al."Identification of a snoRNA47 gene cluster in Oryza sativa".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.5(2002):667-670. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。