Biphasic effect of GABA on rat sperm acrosome reaction: Involvement of GABA(A) and GABA(B) receptors
文献类型：期刊论文
| 作者 | Hu, JH; He, XB; Wu, Q; Yan, YC |
| 刊名 | ARCHIVES OF ANDROLOGY
 |
| 出版日期 | 2002 |
| 卷号 | 48期号:5页码:369-378 |
| 关键词 | baclofen capacitation chlortetracycline staining fertilization saclofen |
| 通讯作者 | Yan, YC (reprint author), Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Mol Cell Biol Lab, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | The functional relationship between GABA(A) and GABA(B) receptors in regulating aerosome reaction (AR) of rat spermatozoa was demonstrated by studying the differential effects of a GABA(B) agonist and an antagonist on the process. AR rates were determined using the chlortetracycline staining assay. The induction of AR in rat sperm by GABA Was found to be a biphasic phenomenon i.e., AR rates increased with increasing GABA concentrations tip to <5 muM and at higher concentrations of the neurotransmitter (>5 muM), there was a reduction in the AR rates. This biphasic phenomenon is apparently due to the differential interaction of the neurotransmitter with GABA receptor subtypes in a dose-dependent manner; i.e., GABA(A) receptors (stimulatory) are primarily activated at low concentration of GABA, while GABA(B) receptors (inhibitory) become activated at higher concentrations. This hypothesis is supported by the present findings that treatment with saclofen, a GABA(B) receptor antagonist, did not influence the AR rates effected by GABA at low concentrations; while the A R rates were maintained at the maximum level at higher concentrations of GABA, resulting in the elimination of the biphasic phenomenon. Baclofen, a GABA(B) receptor agonist, blocks the AR activating action of GABA at both low and high concentrations. It would appear that the induction of AR in rat sperm by GABA is regulated by the proportionality of activated GABA(A) and GABA(B) receptors acting as a yin-yang control. |
| 学科主题 | Endocrinology & Metabolism |
| 类目[WOS] | Andrology |
| 关键词[WOS] | GAMMA-AMINOBUTYRIC-ACID ; MOUSE SPERMATOZOA ; CALCIUM CHANNELS ; MAMMALIAN SPERM ; PLASMA-MEMBRANE ; ZONA-PELLUCIDA ; PROGESTERONE ; CAPACITATION ; TRANSMITTER ; ACTIVATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000177524100007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2508]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hu, JH,He, XB,Wu, Q,et al. Biphasic effect of GABA on rat sperm acrosome reaction: Involvement of GABA(A) and GABA(B) receptors[J]. ARCHIVES OF ANDROLOGY,2002,48(5):369-378. |
| APA | Hu, JH,He, XB,Wu, Q,&Yan, YC.(2002).Biphasic effect of GABA on rat sperm acrosome reaction: Involvement of GABA(A) and GABA(B) receptors.ARCHIVES OF ANDROLOGY,48(5),369-378. |
| MLA | Hu, JH,et al."Biphasic effect of GABA on rat sperm acrosome reaction: Involvement of GABA(A) and GABA(B) receptors".ARCHIVES OF ANDROLOGY 48.5(2002):369-378. |
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