Expression, purification of the recombinant protein fragment of the monkey epididymis expressed gene ESc-615 and preparation of polyclonal antisera to it
文献类型：期刊论文
| 作者 | Hu, ZH; Li, B; Liu, Q; Zhang, YL |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2002 |
| 卷号 | 34期号:6页码:753-756 |
| 关键词 | ESc-615 epididymis recombinant protein expression polyclonal antibody |
| 通讯作者 | , |
| 英文摘要 | ESc-615 is a gene which was specifically expressed in epididymis, newly discovered in our laboratory. To elucidate the function of ESc-615 in sperm maturation and fertilization at the protein level, its C-terminal 310 amino acid residues fragment was expressed in E. coli and used to immunize rabbits to obtain polyclonal antiserum with titer of 100 000. The antisera could detect the antigen as low as 3 ng. A 63 kD protein in rat epididymis protein extracts could be detected in Western blot by using this particular antiserum, in accordance with the size of ESc-615 homologue polypeptide deduced from its cDNA. Immunostaining results showed that ESc-615 was a secreted protein and could bind sperms, which was similar with other carboxylesterases in male reproductive system; the positive staining was eliminated after antigen absorption. All these results showed that this antiserum was not only effective but also highly specific, providing a useful tool in further functional studies of ESc-615. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | MALE REPRODUCTIVE-TRACT |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000179453000014 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2519]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hu, ZH,Li, B,Liu, Q,et al. Expression, purification of the recombinant protein fragment of the monkey epididymis expressed gene ESc-615 and preparation of polyclonal antisera to it[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(6):753-756. |
| APA | Hu, ZH,Li, B,Liu, Q,&Zhang, YL.(2002).Expression, purification of the recombinant protein fragment of the monkey epididymis expressed gene ESc-615 and preparation of polyclonal antisera to it.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(6),753-756. |
| MLA | Hu, ZH,et al."Expression, purification of the recombinant protein fragment of the monkey epididymis expressed gene ESc-615 and preparation of polyclonal antisera to it".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.6(2002):753-756. |
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