Cystatin 11: A new member of the cystatin type 2 family
文献类型：期刊论文
| 作者 | Hamil, KG; Liu, Q; Sivashanmugam, P; Yenugu, S; Soundararajan, R; Grossman, G; Richardson, RT; Zhang, YL; O'Rand, MG; Petrusz, P |
| 刊名 | ENDOCRINOLOGY
 |
| 出版日期 | 2002 |
| 卷号 | 143期号:7页码:2787-2796 |
| 通讯作者 | Hall, SH (reprint author), Univ N Carolina, Reprod Biol Lab, Sch Med, CB 7500, Chapel Hill, NC 27599 USA., |
| 英文摘要 | Cystatin (CST)11, a novel member of the CST type 2 family of cysteine protease inhibitors, was identified in Macaca mulatta epididymis by subtractive hybridization cloning. The human CST11 gene on chromosome 20p11.2 is located near three other CST genes expressed predominantly in the male reproductive tract. The CST11 gene spans three exons, a structure similar to that of other CST family 2 genes. An exon 2-deleted alternative transcript (CST11Delta2) was also identified. CST11 mRNA is expressed only in the epididymis as judged by Northern blot hybridization and is androgen regulated. The protein is most abundant in the initial segment, but is detected throughout the epididymis and on ejaculated human sperm. The calculated tertiary structure of CST11 reveals that the three regions corresponding to the protease inhibitory wedge of CST3 are similarly juxtaposed in CST11, consistent with protease inhibitor function. Intact and exon 2-deleted CST11 recombinant proteins were tested for antibacterial activity. After a 2-h incubation of Escherichia coli with 50 mug/ml recombinant CST11 or CST11Delta2, bacterial colony-forming units were reduced to 30% of control, indicating that both forms have antimicrobial activity. |
| 学科主题 | Endocrinology & Metabolism |
| 类目[WOS] | Endocrinology & Metabolism |
| 关键词[WOS] | 3-DIMENSIONAL SOLUTION STRUCTURE ; CYSTEINE PROTEINASE-INHIBITOR ; RAY CRYSTAL-STRUCTURE ; HERPES-SIMPLEX VIRUS ; CATHEPSIN-L ; PROTEASE INHIBITORS ; SERTOLI CELLS ; MESSENGER-RNA ; CRES GENE ; IN-VITRO |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000176550400041 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2524]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hamil, KG,Liu, Q,Sivashanmugam, P,et al. Cystatin 11: A new member of the cystatin type 2 family[J]. ENDOCRINOLOGY,2002,143(7):2787-2796. |
| APA | Hamil, KG.,Liu, Q.,Sivashanmugam, P.,Yenugu, S.,Soundararajan, R.,...&Hall, SH.(2002).Cystatin 11: A new member of the cystatin type 2 family.ENDOCRINOLOGY,143(7),2787-2796. |
| MLA | Hamil, KG,et al."Cystatin 11: A new member of the cystatin type 2 family".ENDOCRINOLOGY 143.7(2002):2787-2796. |
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