Apomorphine induces trophic factors that support fetal rat mesencephalic dopaminergic neurons in cultures
文献类型：期刊论文
| 作者 | Guo, H; Tang, ZS; Yu, Y; Xu, L; Jin, GZ; Zhou, JW |
| 刊名 | EUROPEAN JOURNAL OF NEUROSCIENCE
 |
| 出版日期 | 2002 |
| 卷号 | 16期号:10页码:1861-1870 |
| 关键词 | apomorphine dopaminergic neurotrophic factors rat tissue culture tyrosine hydroxylase |
| 通讯作者 | Zhou, JW (reprint author), Chinese Acad Sci, Key Lab Proteom, Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Apomorphine, the catechol-derived dopamine D-1/D-2 receptor agonist, is currently in use as an antiparkinsonian drug. It has previously been reported that apomorphine was able to elicit expression of the enzyme tyrosine hydroxylase, a marker for DA neurons, in the fetal rat cerebrocortical cultures whilst in the presence of brain-derived neurotrophic factor. The present study demonstrated that treatment of fetal rat ventral mesencephalic cultures with apomorphine caused a marked increase in the number of dopaminergic neurons. The action of apomorphine can be mimicked by dopamine receptor (D-1 and D-2) agonists or blocked by preincubation with D-1/D-2 receptor antagonists. Incubation of recipient mesencephalic cultures with the conditioned medium derived from apomorphine-stimulated donor mesencephalic cultures elicited a 3.72-fold increase in the number of TH-positive neurons. Increased mRNA expression levels of brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor were also found in the apomorphine-treated mesencephalic cells along with concomitant protein expression increases in the conditioned medium. Moreover, the trophic activity observed could be partially neutralized by antibodies against either brain-derived neurotrophic factor or glial cell line-derived neurotrophic factor. Cultured fetal striatal cells, but not hippocampal cells, also responded to apomorphine treatment. The membrane filtration studies revealed that both <30 kDa and >50 kDa fractions contained trophic activities. The latter characterization distinguishes them from most known neurotrophic factors. These results suggest that the apomorphine-modulated development of dopaminergic neurons may be mediated by activation of the dopamine receptor subtypes D-1 and D-2 thereby increasing the production of multiple growth factors. |
| 学科主题 | Neurosciences & Neurology |
| 类目[WOS] | Neurosciences |
| 关键词[WOS] | FIBROBLAST GROWTH-FACTOR ; TYROSINE-HYDROXYLASE EXPRESSION ; NEUROTROPHIC FACTOR ; PARKINSONS-DISEASE ; SUBSTANTIA-NIGRA ; MESSENGER-RNA ; VENTRAL MESENCEPHALON ; POSTNATAL-DEVELOPMENT ; RETROGRADE TRANSPORT ; PRENATAL-DEVELOPMENT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000179537800004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2532]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Guo, H,Tang, ZS,Yu, Y,et al. Apomorphine induces trophic factors that support fetal rat mesencephalic dopaminergic neurons in cultures[J]. EUROPEAN JOURNAL OF NEUROSCIENCE,2002,16(10):1861-1870. |
| APA | Guo, H,Tang, ZS,Yu, Y,Xu, L,Jin, GZ,&Zhou, JW.(2002).Apomorphine induces trophic factors that support fetal rat mesencephalic dopaminergic neurons in cultures.EUROPEAN JOURNAL OF NEUROSCIENCE,16(10),1861-1870. |
| MLA | Guo, H,et al."Apomorphine induces trophic factors that support fetal rat mesencephalic dopaminergic neurons in cultures".EUROPEAN JOURNAL OF NEUROSCIENCE 16.10(2002):1861-1870. |
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