Association of genetic polymorphisms in CYP2E1, MPO, NQ01, GSTM1, and GSTT1 genes with benzene poisoning
文献类型：期刊论文
| 作者 | Wan, JX; Shi, JX; Hui, LJ; Wu, D; Jin, XP; Zhao, NQ; Huang, W; Xia, ZL; Hu, GX |
| 刊名 | ENVIRONMENTAL HEALTH PERSPECTIVES
 |
| 出版日期 | 2002 |
| 卷号 | 110期号:12页码:1213-1218 |
| 关键词 | benzene poisoning polymorphisms lifestyle NQ01 CYP2E1 MPO GSTM1 GSTT1 |
| 通讯作者 | Hu, GX (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Metabolic enzymes involved in benzene activation or detoxification, including NAD(P)H, quinone oxidoreductase 1 (NQO1), cytochrome P450 2E1 (CYP2E1), myeloperoxidase (MPO), glutathione-S-transferase mu-1 (GSTM1), and glutathione-S-transferase theta-1 (GSTT1), were studied for their roles in human susceptibility to benzene poisoning. The potential interactions of these metabolic enzymes with lifestyle factors such as cigarette smoking and alcohol consumption were also explored. We studied 156 benzene-poisoning patients and 152 workers occupationally exposed to benzene in South China. Sequencing, denaturing HPLC, restriction fragment-length polymorphism, and polymerase chain reaction were used to detect polymorphisms on the promoters and complete coding regions of NQO1, CYP2E1, MPO, and the null genotypes of GSTM1 and GSTT1. Seventeen single nucleotide polymorphisms (SNPs) were identified in NQO1, CYP2E1, and MPO genes, including 6 novel SNPs in CYP2E1 and MPO. Of the subjects who smoked and drank alcohol, an 8.15-fold [95% confidence interval (CI), 1.43-46-50] and a 21-50-fold (95% CI, 2.79-165.79) increased risk of benzene poisoning, respectively, were observed among the subjects with two copies of NQO1 with a C-to-T substitution in cDNA at nucleotide 609 (c.609 C>T variation; i.e., NQO1 c.609 T/T) compared to those with the heterozygous or wild (NQO1 c.609 C/T and c.609 C/C) genotypes. Our data also indicated that individuals with CYP2E1 c.-1293 C/C and c-1293 G/C, and NQO1 c.609 T/T, and GSTT1 null genotypes tended to be more susceptible to benzene toxicity. Our results suggest that the combined effect of polymorphisms in NQO1, CYP2E1, and GSTT1 genes and lifestyle factors might contribute to benzene poisoning. |
| 学科主题 | Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Toxicology |
| 类目[WOS] | Environmental Sciences ; Public, Environmental & Occupational Health ; Toxicology |
| 关键词[WOS] | DT-DIAPHORASE ; BONE-MARROW ; PHENOLIC METABOLITES ; PROGENITOR CELLS ; POTENTIAL ROLE ; TOXICITY ; MUTATION ; IDENTIFICATION ; MYELOTOXICITY ; CHLORZOXAZONE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000179810600024 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2536]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wan, JX,Shi, JX,Hui, LJ,et al. Association of genetic polymorphisms in CYP2E1, MPO, NQ01, GSTM1, and GSTT1 genes with benzene poisoning[J]. ENVIRONMENTAL HEALTH PERSPECTIVES,2002,110(12):1213-1218. |
| APA | Wan, JX.,Shi, JX.,Hui, LJ.,Wu, D.,Jin, XP.,...&Hu, GX.(2002).Association of genetic polymorphisms in CYP2E1, MPO, NQ01, GSTM1, and GSTT1 genes with benzene poisoning.ENVIRONMENTAL HEALTH PERSPECTIVES,110(12),1213-1218. |
| MLA | Wan, JX,et al."Association of genetic polymorphisms in CYP2E1, MPO, NQ01, GSTM1, and GSTT1 genes with benzene poisoning".ENVIRONMENTAL HEALTH PERSPECTIVES 110.12(2002):1213-1218. |
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