Profiling genes differentially expressed in NGX6 overexpressed nasopharyngeal carcinoma cells by cDNA array
文献类型：期刊论文
| 作者 | Ma, J; Li, J; Zhou, J; Li, XL; Tang, K; Zhou, M; Yang, JB; Yan, Q; Shen, SR; Hu, GX |
| 刊名 | JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
 |
| 出版日期 | 2002 |
| 卷号 | 128期号:12页码:683-690 |
| 关键词 | nasopharyngeal carcinoma cDNA array cell cycle gene expression profile |
| 通讯作者 | Li, XL (reprint author), Cent S Univ, Xiang Ya Sch Med, Canc Res Inst, 88 Xiang Ya Rd, Changsha 410078, Hunan, Peoples R China., |
| 英文摘要 | Purpose: To investigate the role of the NGX6 gene in carcinoma proliferation and profile the downstream genes regulated by NGX6 in a nasopharyngeal carcinoma (NPC) cell line. Methods: We established a NPC cell line with NGX6 overexpression by gene transfection. Subsequently, a high-density cDNA array was used to identify differentially expressed genes in NGX6-overxepressed cells. Four differentially expressed genes or EST(expressed sequence tags) were examined using Northern blot. Furthermore, flow cytometry was employed to analyze the percentages of cells in the G(0)-G(1), S, and G(2)-M phase of the cell cycle in a NGX6 overexpression cell line. Results: Fifty-five genes and ESTs were differentially expressed after NGX6 transfection in a cDNA array assay. Several genes related to cell cycle and transcription regulation were identified using this technique. Flow cytometry analysis showed NGX6 overexpression can increase the length of the G(1) phase of the cell cycle in NPC cells. Conclusion: We demonstrated the existence of a panel of genes that can be regulated by NGX6. Overexpression of NGX6 can influence the distribution of the cell cycle in NPC cells. Further studies are necessary to elucidate the exact function of these genes and their relationship to NGX6 expression. |
| 学科主题 | Oncology |
| 类目[WOS] | Oncology |
| 关键词[WOS] | EPSTEIN-BARR-VIRUS ; CHROMOSOMAL LOCATION ; REGULATORY ROLE ; TRANSCRIPTION ; PROTEIN ; IDENTIFICATION ; LOCALIZATION ; MUTATIONS ; COMPLEX ; HOMOLOG |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000180042500007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2537]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Ma, J,Li, J,Zhou, J,et al. Profiling genes differentially expressed in NGX6 overexpressed nasopharyngeal carcinoma cells by cDNA array[J]. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY,2002,128(12):683-690. |
| APA | Ma, J.,Li, J.,Zhou, J.,Li, XL.,Tang, K.,...&Li, GY.(2002).Profiling genes differentially expressed in NGX6 overexpressed nasopharyngeal carcinoma cells by cDNA array.JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY,128(12),683-690. |
| MLA | Ma, J,et al."Profiling genes differentially expressed in NGX6 overexpressed nasopharyngeal carcinoma cells by cDNA array".JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY 128.12(2002):683-690. |
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