beta-Agkistrodotoxin inhibition of Ca2+-dependent release of glutamate, aspartate, glycine and gamma-aminobutyric acid from cerebrocortical synaptosomes following its binding to synaptic membrane
文献类型：期刊论文
| 作者 | Zhang, YQ; Zhou, YC; Shen, GG |
| 刊名 | NEUROREPORT
 |
| 出版日期 | 2002 |
| 卷号 | 13期号:17页码:2313-2317 |
| 关键词 | beta-AgTX binding site synaptosome transmitter release |
| 通讯作者 | Shen, GG (reprint author), Chinese Acad Sci, Shanghai Inst Physiol, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | The binding properties of beta-AgTX, a snake pre-synaptic toxin, membranes and its effect on transmitter release from cerebrocortical synaptosomes were investigated. Assay of [I-125]-beta-AgTX binding to rat synaptic membrane revealed a high affinity binding site for the toxin within the synaptic membrane. Preincubation with beta-AgTX inhibited K+-evoked Ca2+-dependent glutamate release from synaptosomes in a concentration-dependent manner, as determined by an on-line enzyme-linked fluorometric assay. The toxin also blocked the Ca2+-dependent release of other transmitters, aspartate, glycine, and GABA induced by K+-depolarization. However, Ca2+-ionophore, ionomycin-stimulated Ca2+-dependent transmitter release was not significantly affected by beta-AgTX, indicating that the toxin inhibits transmitter release by reducing the entry of Ca2+ into cytoplasm. It is suggested that beta-AgTX-binding site in synaptic membrane is related to the release of a variety of transmitters. |
| 学科主题 | Neurosciences & Neurology |
| 类目[WOS] | Neurosciences |
| 关键词[WOS] | NERVE-TERMINALS ; BOTULINUM ; TETANUS ; NEUROTOXINS ; PROTEIN ; BRAIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000180167700029 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2541]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, YQ,Zhou, YC,Shen, GG. beta-Agkistrodotoxin inhibition of Ca2+-dependent release of glutamate, aspartate, glycine and gamma-aminobutyric acid from cerebrocortical synaptosomes following its binding to synaptic membrane[J]. NEUROREPORT,2002,13(17):2313-2317. |
| APA | Zhang, YQ,Zhou, YC,&Shen, GG.(2002).beta-Agkistrodotoxin inhibition of Ca2+-dependent release of glutamate, aspartate, glycine and gamma-aminobutyric acid from cerebrocortical synaptosomes following its binding to synaptic membrane.NEUROREPORT,13(17),2313-2317. |
| MLA | Zhang, YQ,et al."beta-Agkistrodotoxin inhibition of Ca2+-dependent release of glutamate, aspartate, glycine and gamma-aminobutyric acid from cerebrocortical synaptosomes following its binding to synaptic membrane".NEUROREPORT 13.17(2002):2313-2317. |
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