Interactions between HMG proteins (HMG1/2 and RMG14/17) and human epsilon-globin gene promoter (epsilon-promoter)
文献类型：期刊论文
| 作者 | Hou, CH; Huang, J; Zhang, SB; Qian, RL |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2002 |
| 卷号 | 47期号:18页码:1543-1546 |
| 关键词 | HNIG proteins human epsilon-globin gene promoter in vitro nueleosome reconstitution HMG proteins purification EMSA |
| 通讯作者 | Qian, RL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | High mobility group (HMG) proteins are abundant non-histone proteins in the nuclei of eukaryocytes. It has been shown that HMG proteins may play important roles in the structure and function of chromatin. In the present study, the binding of HMG proteins (HMG1/2 and HMG14/17) to the human e-globin gene promoter (e-promoter, -177-+1 bp) has been examined by using both the in vitro nucleosome reconstitution and the electrophoresis mobility shift assay (EMSA). We found that HMG1/2 proteins could bind to the naked E-promoter DNA, however, HMG14/17 could not. Using the in vitro nucleosome reconstitution, we revealed that HMG14/17 could bind to the mononucleosome reconstituted in vitro with E-promoter, while HMG1/2 could not. Those results indicate that the binding of HMG proteins to c-promoter is dynamic as the nucleosome assembling and disassembling. We speculated that this selective binding of HMG proteins to E-promoter might play a critical role in the regulation of E-globin gene expression. |
| 学科主题 | Science & Technology - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 关键词[WOS] | TATA-BINDING PROTEIN ; CIS-ACTING ELEMENTS ; CHROMOSOMAL-PROTEINS ; TRANSCRIPTION ; NUCLEOSOMES ; ACTIVATION ; SEQUENCE ; INVITRO ; DOMAIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000178124100011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2542]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hou, CH,Huang, J,Zhang, SB,et al. Interactions between HMG proteins (HMG1/2 and RMG14/17) and human epsilon-globin gene promoter (epsilon-promoter)[J]. CHINESE SCIENCE BULLETIN,2002,47(18):1543-1546. |
| APA | Hou, CH,Huang, J,Zhang, SB,&Qian, RL.(2002).Interactions between HMG proteins (HMG1/2 and RMG14/17) and human epsilon-globin gene promoter (epsilon-promoter).CHINESE SCIENCE BULLETIN,47(18),1543-1546. |
| MLA | Hou, CH,et al."Interactions between HMG proteins (HMG1/2 and RMG14/17) and human epsilon-globin gene promoter (epsilon-promoter)".CHINESE SCIENCE BULLETIN 47.18(2002):1543-1546. |
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