Enhanced thiosulfate-silver staining of proteins in gels using Coomassie Blue and chromium
文献类型：期刊论文
| 作者 | Zhou, ZD; Liu, WY; Li, MQ |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2002 |
| 卷号 | 24期号:19页码:1561-1567 |
| 关键词 | chromium sulfate Coomassie Brilliant Blue R-250 protein sensitivity enhancer proteomics thiosulfate-silver staining |
| 通讯作者 | Li, MQ (reprint author), Chinese Acad Sci, Shanghai Inst Nucl Res, Lab Nanobiol & Nanomed, 2019 Jia Luo Rd, Shanghai 201800, Peoples R China., |
| 英文摘要 | Potassium chromium sulfate, a new sensitivity enhancer for silver staining of proteins in gels, enhanced the sensitivity of the thiosulfate-silver staining method. The sensitivity could be further improved when potassium chromium sulfate was used in association with another sensitivity enhancer, Coomassie Brilliant Blue R-250 (CBB R-250). The sensitivity of the CBB-chromium modified method to strongly basic proteins such as ribosomal proteins was about 20-fold over that of the published method. This novel method has direct applicability for 2-D gel electrophoresis used in proteomics. |
| 学科主题 | Biotechnology & Applied Microbiology |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | POLYACRYLAMIDE GELS ; RIBOSOMAL-PROTEINS ; DETECTING PROTEINS ; ELECTROPHORESIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000178207100006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2543]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhou, ZD,Liu, WY,Li, MQ. Enhanced thiosulfate-silver staining of proteins in gels using Coomassie Blue and chromium[J]. BIOTECHNOLOGY LETTERS,2002,24(19):1561-1567. |
| APA | Zhou, ZD,Liu, WY,&Li, MQ.(2002).Enhanced thiosulfate-silver staining of proteins in gels using Coomassie Blue and chromium.BIOTECHNOLOGY LETTERS,24(19),1561-1567. |
| MLA | Zhou, ZD,et al."Enhanced thiosulfate-silver staining of proteins in gels using Coomassie Blue and chromium".BIOTECHNOLOGY LETTERS 24.19(2002):1561-1567. |
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