Isolation and enzymatic characterization of lamjapin, the first ribosome-inactivating protein from cryptogamic algal plant (Laminaria japonica A)
文献类型：期刊论文
| 作者 | Liu, RS; Yang, JH; Liu, WY |
| 刊名 | EUROPEAN JOURNAL OF BIOCHEMISTRY
 |
| 出版日期 | 2002 |
| 卷号 | 269期号:19页码:4746-4752 |
| 关键词 | kelp lamjapin marine alga ribosome-inactivating protein RNA N-glycosidase |
| 通讯作者 | Liu, WY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Lamjapin, a novel type I ribosome-inactivating protein, has been isolated from kelp (Laminaria japonica A), a marine alga. This protein has been extensively purified through multiple chromatography columns. With a molecular mass of approximate to 36 kDa, lamjapin is slightly larger than the other known single-chain ribosome-inactivating proteins from the higher plants. Lamjapin can inhibit protein synthesis in rabbit reticulocyte lysate with an IC50 of 0.69 nm. It can depurinate at multiple sites of RNA in rat ribosome and produce the diagnostic R-fragment and three additional larger fragments after the aniline reaction. Lamjapin can deadenylate specifically at the site A20 of the synthetic oligoribonucleotide (35-mer) substrate that mimics the sarcin/ricin domain (SRD) of rat ribosomal 28S RNA. However, it cannot hydrolyze the N-C glycosidic bond of guanosine, cytidine or uridine at the corresponding site of the A20 of three mutant SRD RNAs. Lamjapin exhibits the same base and position requirement as the ribosome-inactivating proteins from higher plants. We conclude that lamjapin is an RNA N-glycosidase that belongs to the ribosome-inactivating protein family. This study reports for the first time that ribosome-inactivating protein exists in the lower cryptogamic algal plant. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | RICIN A-CHAIN ; GLYCOSIDASE ACTIVITY ; RNA ; RECOGNITION ; MECHANISM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000178275200004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2544]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, RS,Yang, JH,Liu, WY. Isolation and enzymatic characterization of lamjapin, the first ribosome-inactivating protein from cryptogamic algal plant (Laminaria japonica A)[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY,2002,269(19):4746-4752. |
| APA | Liu, RS,Yang, JH,&Liu, WY.(2002).Isolation and enzymatic characterization of lamjapin, the first ribosome-inactivating protein from cryptogamic algal plant (Laminaria japonica A).EUROPEAN JOURNAL OF BIOCHEMISTRY,269(19),4746-4752. |
| MLA | Liu, RS,et al."Isolation and enzymatic characterization of lamjapin, the first ribosome-inactivating protein from cryptogamic algal plant (Laminaria japonica A)".EUROPEAN JOURNAL OF BIOCHEMISTRY 269.19(2002):4746-4752. |
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