Repression through a distal TCF-3 binding site restricts Xenopus myf-5 expression in gastrula mesoderm
文献类型：期刊论文
| 作者 | Yang, J; Mei, WY; Otto, A; Xiao, L; Tao, QH; Geng, X; Rupp, RAW; Ding, XY |
| 刊名 | MECHANISMS OF DEVELOPMENT
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| 出版日期 | 2002 |
| 卷号 | 115期号:1页码:79-89 |
| 关键词 | Xenopus myogenesis myf-5 TCF restriction enzyme mediated integration transgenesis mesoderm patterning Wnt signal transforming growth factor beta |
| 通讯作者 | Ding, XY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | The development of skeletal muscle in the vertebrate embryo is controlled by a transcriptional cascade that includes the four myogenic regulatory factors Myf-5, MyoD, Myogenin, and MRF4. The dynamic expression pattern of myf-5 during myogenesis is thought to be consistent with its role during early determination of the myogenic lineage. To study the factors and mechanisms, which regulate myf-5 transcription in Xenopus, we isolated a genomic DNA clone containing 4858 bp of Xmyf-5 5' flanking region. Using a transgenic reporter assay, we show here that this genomic contig is sufficient to recapitulate the dynamic stage- and tissue-specific expression pattern of Xmyf-5 from the gastrula to tail bud stages. For the primary induction of myf-5 transcription, we identify three main regulatory elements, which are responsible for (i) activation in dorsal mesoderm, (ii) activation in ventral mesoderm, and (iii) repression in midline mesoderm, respectively. Their combined activities define the two-winged expression domain of myf-5 in the preinvoluted mesoderm. Repression in midline mesoderm is mediated by a single TCF binding site located in the 5' end of the -4.8 kbp sequence, which binds XTcf-3 protein in vitro. Endogenous Wnt signaling in the lateral mesoderm is required to overcome the long-range repression through this distal TCF site, and to stimulate myf-5 transcription independently from it. The element for ventral mesoderm activation responds to Activin. Together, these results describe a regulatory mosaic of repression and activation, which defines the myf-5 expression profile in the frog gastrula. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved. |
| 学科主题 | Developmental Biology |
| 类目[WOS] | Developmental Biology |
| 关键词[WOS] | SKELETAL-MUSCLE ; SPEMANNS ORGANIZER ; GENE MYF-5 ; EMBRYOS ; CELLS ; MYOD ; TRANSCRIPTION ; MYOGENESIS ; INDUCTION ; WNT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000176530100008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2553]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yang, J,Mei, WY,Otto, A,et al. Repression through a distal TCF-3 binding site restricts Xenopus myf-5 expression in gastrula mesoderm[J]. MECHANISMS OF DEVELOPMENT,2002,115(1):79-89. |
| APA | Yang, J.,Mei, WY.,Otto, A.,Xiao, L.,Tao, QH.,...&Ding, XY.(2002).Repression through a distal TCF-3 binding site restricts Xenopus myf-5 expression in gastrula mesoderm.MECHANISMS OF DEVELOPMENT,115(1),79-89. |
| MLA | Yang, J,et al."Repression through a distal TCF-3 binding site restricts Xenopus myf-5 expression in gastrula mesoderm".MECHANISMS OF DEVELOPMENT 115.1(2002):79-89. |
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