Studies of three genes encoding Cinnamomin (a type II RIP) isolated from the seeds of camphor tree and their expression patterns
文献类型：期刊论文
| 作者 | Yang, Q; Liu, RS; Gong, ZZ; Liu, WY |
| 刊名 | GENE
 |
| 出版日期 | 2002 |
| 卷号 | 284期号:1页码:215-223 |
| 关键词 | cotyledons N-glycosylation sites ribosome-inactivating protein sequence identity gene expression |
| 通讯作者 | Liu, WY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Cinnamomin, which has three isoforms, is a type II ribosome-inactivating protein (RIP) purified from the mature seeds of camphor tree (Cinnamomum camphora). In a previous study, an incomplete cDNA that encoded the A- and B-chain of Cinnamomin but lacked signal peptide sequence was cloned. In the present paper, its full-length cDNA was obtained by 5' rapid amplification of cDNA ends (5'RACE). Subsequently, polymerase chain reaction (PCR) amplification of its genomic DNA was performed. Unexpectedly, sequence analysis of the PCR products revealed three cinnamomin genes with >98.0% sequence identity. One of them corresponded to the published cDNA and was designated as cinnamomin I, whereas the other two genes were named as cinnamomin II and cinnamomin III, respectively. RT-PCR amplification of the cDNAs of cinnamomin II and III manifested that these two genes were functional. The three genes have no intron. Three Cinnamomin precursors that were inferred from the cDNA sequence of three cinnamomin genes exhibited relatively high sequence homology with other type II RIPs. Northern blot analysis demonstrated that the cinnamomin genes only expressed in cotyledons of C. camphora seeds and the acmes of expression emerged at 75-90 DAF when seeds were close to maturity. It is proposed that the three cinnamomin genes may encode three isoforms of Cinnamomin. The physiological function of Cinnamomin in C. camphora seeds is briefly discussed. (C) 2002 Elsevier Science B.V. All rights reserved. |
| 学科主题 | Genetics & Heredity |
| 类目[WOS] | Genetics & Heredity |
| 关键词[WOS] | RIBOSOME-INACTIVATING PROTEINS ; A-CHAIN ; MECHANISM ; CLONING ; LECTIN ; 2.5-A ; RNA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000174598100021 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2555]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yang, Q,Liu, RS,Gong, ZZ,et al. Studies of three genes encoding Cinnamomin (a type II RIP) isolated from the seeds of camphor tree and their expression patterns[J]. GENE,2002,284(1):215-223. |
| APA | Yang, Q,Liu, RS,Gong, ZZ,&Liu, WY.(2002).Studies of three genes encoding Cinnamomin (a type II RIP) isolated from the seeds of camphor tree and their expression patterns.GENE,284(1),215-223. |
| MLA | Yang, Q,et al."Studies of three genes encoding Cinnamomin (a type II RIP) isolated from the seeds of camphor tree and their expression patterns".GENE 284.1(2002):215-223. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。