A mRNA molecule encoding truncated excitatory amino acid carrier 1 (EAAC1) protein (EAAC2) is transcribed from an independent promoter but not an alternative splicing event
文献类型：期刊论文
| 作者 | Jin, XP; Bin Peng, J; Huang, F; Zhu, YN; Fei, J; Guo, LH |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2002 |
| 卷号 | 12期号:1页码:257-262 |
| 关键词 | genomic organization glutamate transporter transcriptional start sites EAAC1 |
| 通讯作者 | Guo, LH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Lab Mol Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Glutamate transporter EAAC1 removes excitatory neurotransmitter in central nervous system, and also absorbs glutamate in epithelia of intestine, kidney, liver and heart for normal cell growth. When a mouse cDNA was screened using EAAC1 cDNA fragment as probe in our lab, a transcript (GenBank U75214) encoding an EAAC1 protein with 148 residues truncated at N-terminal was cloned and named as EAAC2. Sequence analysis shows that EAAC2 has it's own start code and unique 5'UTR that is different from that of EAAC1. A mouse genomic library was screened and a positive clone including EAAC1 CDS was sequenced (GenBank AF 322393) and indicates that normal EAAC1 transcript (GenBank U73521) is transcribed from 10 exons in terms of exon I, II, III, IV, V, VI, VII, VIII, IX, X, and EAAC2 transcript is consisted by exons from IV to IX as same as that of EAAC1 and with its unique exon beta upstream to exon IV and exon delta downstream to IX. EAAC2 transcript has a cluster of transcriptional start sites not overlapping with the transcriptional start sites of EAAC1. These results indicate that EAAC2 is transcribed from an independent promoter but not an alternative splicing event. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | AFFINITY GLUTAMATE TRANSPORTER ; DICARBOXYLIC AMINOACIDURIA ; RAT-BRAIN ; EXPRESSION ; CLONING ; SUBTYPES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000178031600010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2558]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Jin, XP,Bin Peng, J,Huang, F,et al. A mRNA molecule encoding truncated excitatory amino acid carrier 1 (EAAC1) protein (EAAC2) is transcribed from an independent promoter but not an alternative splicing event[J]. CELL RESEARCH,2002,12(1):257-262. |
| APA | Jin, XP,Bin Peng, J,Huang, F,Zhu, YN,Fei, J,&Guo, LH.(2002).A mRNA molecule encoding truncated excitatory amino acid carrier 1 (EAAC1) protein (EAAC2) is transcribed from an independent promoter but not an alternative splicing event.CELL RESEARCH,12(1),257-262. |
| MLA | Jin, XP,et al."A mRNA molecule encoding truncated excitatory amino acid carrier 1 (EAAC1) protein (EAAC2) is transcribed from an independent promoter but not an alternative splicing event".CELL RESEARCH 12.1(2002):257-262. |
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