Cloning and characterization of a mouse liver-specific gene mfrep-1, upregulated in liver regeneration
文献类型：期刊论文
| 作者 | Yan, J; Ying, H; Gu, F; He, J; Li, YL; Liu, HM; Xu, YH |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2002 |
| 卷号 | 12期号:1页码:353-361 |
| 关键词 | mfrep-1 in silico cloning liver regeneration liver-specific expression |
| 通讯作者 | Xu, YH (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Lab Mol & Cellular Oncol, Shanghai Inst Biol Sci, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,yhxu@sunm.shcnc.ac.cn |
| 英文摘要 | Human fibrinogen-related protein-1/liver fibrinogen-related protein-1 (HFREP-1/LFIRE-1), a liver-specific protein, is a member of fibrinogen superfamily that exerts various biological activities. However, the function of HFREP-1/LFIRE-1 in liver remains unknown. Here we isolated its mouse ortholog gene-mouse fibrinogen-related protein-1 (mfrep-1), which encoded 314 amino acids, exhibiting 80.4% similarity to HFREP-1/LFIRE-1. Northern blot analysis revealed that 1.2-kb mfrep-1 mRNA was detected selectively in mouse liver. To explore the function of MFREP-1, we examined the levels of mfrep-1 mRNA during regeneration after 70% partial hepatectomy (PHx) in mice. mfrep-1 mRNA increased in the regenerating liver and reached the first shoulder peak at 2-4 h after PHx. Cycloheximide pretreatment could suppress the induction of mfrep-1, indicating the up-regulation of this gene need de novo protein synthesis. Its mRNA continued to elevate at 6 h thereafter and reached the second peak at 24 h. The enhanced expression of mfrep-1 maintained high until 72 h and then declined slowly to the basal level. Immunohistochemistry assessment confirmed the up-regulated expression of MFREP-1 protein in parenchymal cells during liver regeneration. These data suggested that MFREP-1 might play an important role in liver regeneration and be involved in the regulation of cell growth. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | PARTIAL-HEPATECTOMY ; EXPRESSION ; GROWTH ; RAT ; IDENTIFICATION ; INJURY ; MICE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000180476800005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2562]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yan, J,Ying, H,Gu, F,et al. Cloning and characterization of a mouse liver-specific gene mfrep-1, upregulated in liver regeneration[J]. CELL RESEARCH,2002,12(1):353-361. |
| APA | Yan, J.,Ying, H.,Gu, F.,He, J.,Li, YL.,...&Xu, YH.(2002).Cloning and characterization of a mouse liver-specific gene mfrep-1, upregulated in liver regeneration.CELL RESEARCH,12(1),353-361. |
| MLA | Yan, J,et al."Cloning and characterization of a mouse liver-specific gene mfrep-1, upregulated in liver regeneration".CELL RESEARCH 12.1(2002):353-361. |
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