The interaction between the human beta-globin locus control region and nuclear matrix
文献类型：期刊论文
| 作者 | Zhang, SB; Qian, RL |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2002 |
| 卷号 | 12期号:1页码:411-416 |
| 关键词 | human beta-globin LCR nuclear matrix proteins nuclear matrix HEL cells |
| 通讯作者 | Qian, RL (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 Yueyang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Our previous study showed that hydroxyurea (Hu) could induce HEL cells to express human beta-globin gene. However the molecular mechanisms by which the expression of beta-globin gene is activated and regulated are poorly understood. Here we show that the binding patterns between the core DNA sequences (HS2 core sequence -10681similar to -10971 bp, HS3 core sequence -14991similar to -14716 bp and HS4 core sequence -18586similar to -18306 bp) of DNase I hypersensitive sites in the human beta-globin LCR and nuclear matrix proteins isolated from Hu induced and uninduced HEL cells are quite different. Results demonstrated that nuclear matrix proteins might play important roles in regulating the expression of human beta-like globin genes through their interaction with HSs (HS2,HS3 and HS4 core sequences) in the LCR. Moreover, the results obtained from the in vitro DNA-matrix binding assay showed that the core DNA sequences of DNase I hypersensitive sites (HS2, HS3 and HS4) were unable to bind to the nuclear matrix isolated from uninduced HEL cells; in addition, HS2 core DNA sequence was capable of binding to the nuclear matrix prepared from Hu-induced HEL cells, while both HS3 and HS4 core DNA sequences could not do so. Results indicated that the HS2 core DNA sequence may be a functional MAR (matrix attachment region). We suggest that the HS2 core DNA sequence binding to the nuclear matrix in Hu-induced HEL cells may open the structure of chromatin to make the LCR accessible to the promoter of beta-globin gene and to promote its transcription. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | PROTEIN ; GENE ; EXPRESSION ; BINDING |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000180476800012 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2565]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, SB,Qian, RL. The interaction between the human beta-globin locus control region and nuclear matrix[J]. CELL RESEARCH,2002,12(1):411-416. |
| APA | Zhang, SB,&Qian, RL.(2002).The interaction between the human beta-globin locus control region and nuclear matrix.CELL RESEARCH,12(1),411-416. |
| MLA | Zhang, SB,et al."The interaction between the human beta-globin locus control region and nuclear matrix".CELL RESEARCH 12.1(2002):411-416. |
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