NC1 domain of human type VIII collagen (alpha 1) inhibits bovine aortic endothelial cell proliferation and causes cell apoptosis
文献类型：期刊论文
| 作者 | Xu, R; Yao, ZY; Xin, L; Zhang, Q; Li, TP; Gan, RB |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2001 |
| 卷号 | 289期号:1页码:264-268 |
| 关键词 | vastatin type VIII collagen NC1 domain angiogenesis inhibitor apoptosis |
| 通讯作者 | Gan, RB (reprint author), Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Inst Biochem & Cell Biol, 320 Yue Yang Rd,POB 55, Shanghai 200031, Peoples R China., |
| 英文摘要 | Endostatin, a natural angiogenesis inhibitor, had been identified for years. It opened a new approach for cancer therapy. Sequence analysis reveled that endostatin is the NC1 domain (non-triple-helical domain) of collagen XVIII. In this report, the cDNA of NC1 domain of type VIII collagen (alpha 1) was cloned and expressed as soluble form in Escherichia coli. The recombinant protein was purified with Ni-NTA agarose column and named as vastatin. It inhibited the proliferation of bovine aortic endothelial (BAE) cell stimulated by basic fibroblast growth factor (bFGF) in a dose-dependent manner. The ED50 of vastatin was 0.6 mug/ml, while the ED50 of endostatin was 0.5 mug/ml. Treatment of BAE cell with vastatin caused G(0)-G(1) arrest and cell apoptosis. It is interesting that sequence analysis showed that there was only about 12% amino acid sequence homology between vastatin and endostatin. The structure-function relationship of these angiogenesis molecules remains to be elucidated. (C) 2001 Academic Press. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | TUMOR-GROWTH ; ENDOSTATIN ; ANGIOGENESIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000172451100044 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2572]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xu, R,Yao, ZY,Xin, L,et al. NC1 domain of human type VIII collagen (alpha 1) inhibits bovine aortic endothelial cell proliferation and causes cell apoptosis[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2001,289(1):264-268. |
| APA | Xu, R,Yao, ZY,Xin, L,Zhang, Q,Li, TP,&Gan, RB.(2001).NC1 domain of human type VIII collagen (alpha 1) inhibits bovine aortic endothelial cell proliferation and causes cell apoptosis.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,289(1),264-268. |
| MLA | Xu, R,et al."NC1 domain of human type VIII collagen (alpha 1) inhibits bovine aortic endothelial cell proliferation and causes cell apoptosis".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 289.1(2001):264-268. |
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