Partial characterization of the N-linked oligosaccharide structures on P-selectin glycoprotein ligand-1 (PSGL-1)
文献类型：期刊论文
| 作者 | Aeed, PA; Geng, JG; Asa, D; Raycroft, L; Ma, L; Elhammer, AP |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2001 |
| 卷号 | 11期号:1页码:28-36 |
| 关键词 | selectin ligands PSGL-1 N-glycans |
| 通讯作者 | Elhammer, AP (reprint author), Pharmacia Corp, 7000 Portage Rd, Kalamazoo, MI 49001 USA.,ake.elhammer@pharmacia.com |
| 英文摘要 | PSGL-1, a specific ligand for P-, E and L-selectin, was isolated from in vivo [H-3]-glucosamine labeled HL-60 cells by a combination of wheat germ agglutinin-agarose and P- or E-selectin-agarose chromatography. N-linked oligosaccharides were released fi om the purified, denatured ligand molecule by peptide: N-glycosidase F treatment and, following separation by Sephacryl S-200 chromatography, partially characterized using lectin, ion-exchange and size-exclusion chromatography in combination with glycosidase digestions. The data obtained suggest that the N-glycans on PSGL-1 are predominantly core-fucosylated, multiantennary complex type structures with extended, poly-N-acetyllactosamine containing outer chains. A portion of the outer chains appears to be substituted with fucose indicating that the N-glycans, in addition to the O-glycans on PSGL-1, may be involved in selectin binding. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | MYELOID CELLS ; AMINO-TERMINUS ; GROWTH-FACTOR ; O-GLYCANS ; LECTIN ; NEUTROPHILS ; SPECIFICITY ; LEUKOCYTES ; EXPRESSION ; CONSENSUS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000167901800004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2578]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Aeed, PA,Geng, JG,Asa, D,et al. Partial characterization of the N-linked oligosaccharide structures on P-selectin glycoprotein ligand-1 (PSGL-1)[J]. CELL RESEARCH,2001,11(1):28-36. |
| APA | Aeed, PA,Geng, JG,Asa, D,Raycroft, L,Ma, L,&Elhammer, AP.(2001).Partial characterization of the N-linked oligosaccharide structures on P-selectin glycoprotein ligand-1 (PSGL-1).CELL RESEARCH,11(1),28-36. |
| MLA | Aeed, PA,et al."Partial characterization of the N-linked oligosaccharide structures on P-selectin glycoprotein ligand-1 (PSGL-1)".CELL RESEARCH 11.1(2001):28-36. |
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