In vitro triplex formation and functional analysis of TFOs designed against human c-sis/PDGF-B proto-oncogene
文献类型：期刊论文
| 作者 | Liu, J; Xu, RH; Jin, YX; Wang, DB |
| 刊名 | SCIENCE IN CHINA SERIES C-LIFE SCIENCES
 |
| 出版日期 | 2001 |
| 卷号 | 44期号:1页码:83-91 |
| 关键词 | c-sis/PDGF-B proto-oncogene tripler-forming oligonucleotides (TFO) |
| 通讯作者 | Jin, YX (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | PDGF (platelet derived growth factor) has been shown to play an important role in tumorigenesis, tumor growth, atherosclerosis and inflammation and other various pathologic settings. PDGF-B chain gene is 92% homologous to v-sis oncogene of the simian sarcoma virus. Thus PDGF-B gene is also called c-sis proto-oncogene. This report provides 3 TFOs (triplex-forming oligonucleotides) to inhibit the expression of c-sis/PDGF-B gene. The results from gel mobility shift analysis, in vitro transcription, DNase I footprinting and protein binding assays demonstrate that the TFOs we designed can form sequence-specific stable tripler with the target, and can effectively suppress the downstream gene transcription and inhibit transcription factors binding. They can be used for preparation of drugs to inhibit tumor growth and for the therapy of atherosclerosis, inflammation, etc. |
| 学科主题 | Life Sciences & Biomedicine - Other Topics |
| 类目[WOS] | Biology |
| 关键词[WOS] | OSTEOSARCOMA CELLS ; GENE ; TRANSCRIPTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000167665600011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2581]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, J,Xu, RH,Jin, YX,et al. In vitro triplex formation and functional analysis of TFOs designed against human c-sis/PDGF-B proto-oncogene[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2001,44(1):83-91. |
| APA | Liu, J,Xu, RH,Jin, YX,&Wang, DB.(2001).In vitro triplex formation and functional analysis of TFOs designed against human c-sis/PDGF-B proto-oncogene.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,44(1),83-91. |
| MLA | Liu, J,et al."In vitro triplex formation and functional analysis of TFOs designed against human c-sis/PDGF-B proto-oncogene".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 44.1(2001):83-91. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。