中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Binding of human cardiac myosin light chain 1 to heavy chain and actin

文献类型:期刊论文

作者Huang, RJ; Peng, BZ; Zhou, GY; Shen, JY; Gong, ZX
刊名ACTA BIOCHIMICA ET BIOPHYSICA SINICA
出版日期2001
卷号33期号:1页码:41-45
关键词binding human cardiac myosin light chain 1 cardiac myosin heavy chain actin monoclonal antibody
通讯作者,
英文摘要The cloning and sequence analysis of Chinese human cardiac myosin light chain 1 (CCMLC1) was previously reported. In this paper the cDNA of CCMLC1 was used as template and both of cDNAs of N and C terminal fragments of CCMLC1, each containing 98 amino acid residues, were obtained by PCR. Using the expressed products of both fragments, the binding experiments of two fragments to cardiac myosin heavy chain of rat, human cardiac actin and to monoclonal antibody raised against CCMLC1, have been performed, respectively, by means of precipitation with GST-Sepharose beads. The results showed that all the heavy chain, actin and monoclonal antibody bound the N terminal fragment of CCMLC1 at different sites. Under experimental conditions, the binding of CCMLC1 with actin could affect the subsequent binding of CCMLC1 to heavy chain in topologically.
学科主题Biochemistry & Molecular Biology; Biophysics
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]GENE ; MUSCLE
收录类别SCI
语种中文
WOS记录号WOS:000166598200008
版本出版稿
源URL[http://202.127.25.143/handle/331003/2584]  
专题上海生化细胞研究所_上海生科院生化细胞研究所
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GB/T 7714
Huang, RJ,Peng, BZ,Zhou, GY,et al. Binding of human cardiac myosin light chain 1 to heavy chain and actin[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2001,33(1):41-45.
APA Huang, RJ,Peng, BZ,Zhou, GY,Shen, JY,&Gong, ZX.(2001).Binding of human cardiac myosin light chain 1 to heavy chain and actin.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,33(1),41-45.
MLA Huang, RJ,et al."Binding of human cardiac myosin light chain 1 to heavy chain and actin".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 33.1(2001):41-45.

入库方式: OAI收割

来源:上海生物化学与细胞生物学研究所

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