Binding of human cardiac myosin light chain 1 to heavy chain and actin
文献类型：期刊论文
| 作者 | Huang, RJ; Peng, BZ; Zhou, GY; Shen, JY; Gong, ZX |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2001 |
| 卷号 | 33期号:1页码:41-45 |
| 关键词 | binding human cardiac myosin light chain 1 cardiac myosin heavy chain actin monoclonal antibody |
| 通讯作者 | , |
| 英文摘要 | The cloning and sequence analysis of Chinese human cardiac myosin light chain 1 (CCMLC1) was previously reported. In this paper the cDNA of CCMLC1 was used as template and both of cDNAs of N and C terminal fragments of CCMLC1, each containing 98 amino acid residues, were obtained by PCR. Using the expressed products of both fragments, the binding experiments of two fragments to cardiac myosin heavy chain of rat, human cardiac actin and to monoclonal antibody raised against CCMLC1, have been performed, respectively, by means of precipitation with GST-Sepharose beads. The results showed that all the heavy chain, actin and monoclonal antibody bound the N terminal fragment of CCMLC1 at different sites. Under experimental conditions, the binding of CCMLC1 with actin could affect the subsequent binding of CCMLC1 to heavy chain in topologically. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | GENE ; MUSCLE |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000166598200008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2584]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Huang, RJ,Peng, BZ,Zhou, GY,et al. Binding of human cardiac myosin light chain 1 to heavy chain and actin[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2001,33(1):41-45. |
| APA | Huang, RJ,Peng, BZ,Zhou, GY,Shen, JY,&Gong, ZX.(2001).Binding of human cardiac myosin light chain 1 to heavy chain and actin.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,33(1),41-45. |
| MLA | Huang, RJ,et al."Binding of human cardiac myosin light chain 1 to heavy chain and actin".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 33.1(2001):41-45. |
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