中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Altering fibroin heavy chain gene of silkworm Bombyx mori by homologous recombination

文献类型:期刊论文

作者Zhao, Y; Chen, X; Peng, WP; Dong, L; Huang, JT; Lu, CD
刊名ACTA BIOCHIMICA ET BIOPHYSICA SINICA
出版日期2001
卷号33期号:1页码:112-116
关键词silkworm (Bombyx mori) GFP synthetic fibroin-like gene transgenic homologous recombination
通讯作者,
英文摘要A gene unit, which encoded fibroin-like peptide, was synthesized and constructed. The unit was multimerized to about 2 400 bp using BamHI and BglII at each end of the unit, then was fused with gfp reporter gene. The fusion gene, flanked by the 5' and 3' sequence of the fibroin heavy chain gene of silkworm Bombyx: mori, was transferred into the eggs of silkworm by electroporation. After the silkworms developed and spinned silk, 73 out of about 5 400 cocoons were brighter than normal ones under UV light. The protein extracted from the brighter cocoon could react with the GFP polyclonal antibodies. Genomic DNA from these silkworms and their progenies were analyzed. The integration of gfp gene into genomic DNA of silkworm and the occurrence of expected homologous recombination event had been proved by Southern hybridization. It was shown that gfp-fibroin like fusion gene had integrated into the genomic DNA of silkworm by homologous recombination and the phenotype of "brighter cocoon" could be used to select transgenic silkworms.
学科主题Biochemistry & Molecular Biology; Biophysics
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]SPIDER DRAGLINE SILK ; TRANSGENIC SILKWORM
收录类别SCI
语种中文
WOS记录号WOS:000166598200020
版本出版稿
源URL[http://202.127.25.143/handle/331003/2587]  
专题上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式
GB/T 7714
Zhao, Y,Chen, X,Peng, WP,et al. Altering fibroin heavy chain gene of silkworm Bombyx mori by homologous recombination[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2001,33(1):112-116.
APA Zhao, Y,Chen, X,Peng, WP,Dong, L,Huang, JT,&Lu, CD.(2001).Altering fibroin heavy chain gene of silkworm Bombyx mori by homologous recombination.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,33(1),112-116.
MLA Zhao, Y,et al."Altering fibroin heavy chain gene of silkworm Bombyx mori by homologous recombination".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 33.1(2001):112-116.

入库方式: OAI收割

来源:上海生物化学与细胞生物学研究所

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