Treatment of Parkinson's disease by genetically modified immortalized fibroblasts
文献类型：期刊论文
| 作者 | Xu, DH; Chen, XW; Xu, LF; Cao, L; Chen, SD; Liu, XY; Zheng, ZC |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2001 |
| 卷号 | 33期号:1页码:117-122 |
| 关键词 | Parkinson's disease fibroblasts SV40LT antigen gene TH gene |
| 通讯作者 | , |
| 英文摘要 | Primary rat fibroblast cells were immortalized by genetic modification of SV40 large T antigen (LTAg) gene and called RFLT. This cell line was non-tumorigenic after grafting into nud-mouse and rat. The LTAg gene stopped expression when the cells were transplanted in rat striatum, but it resumed the expression ability after the transplanted cells were recovered from striatum and cultured in vitro. TH gene and GCH gene were transfected into RFLT, respectively, and two types of transfected cells, RFLT-TH and RFLT-GCH, were obtained. In mixed culture with these two cell lines, DA was detected with HPLC-ECD. Implanting mixture of those cells into the striatum of PD rats significantly decreased their rotational asymmetry for up to 12 weeks. The expression of TH gene was proved by TH immunohistochemical staining in the sections of rat brain. The establishment of the genetically modified immortalized cells may play role in the gene therapy of PD. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | T-ANTIGEN GENE ; CELLS ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000166598200021 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2588]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xu, DH,Chen, XW,Xu, LF,et al. Treatment of Parkinson's disease by genetically modified immortalized fibroblasts[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2001,33(1):117-122. |
| APA | Xu, DH.,Chen, XW.,Xu, LF.,Cao, L.,Chen, SD.,...&Zheng, ZC.(2001).Treatment of Parkinson's disease by genetically modified immortalized fibroblasts.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,33(1),117-122. |
| MLA | Xu, DH,et al."Treatment of Parkinson's disease by genetically modified immortalized fibroblasts".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 33.1(2001):117-122. |
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