Contribution of the residue Glu9, Glu46, and Phe49 to the biological activity of insulin-like growth factor-1
文献类型：期刊论文
| 作者 | Chen, H; Feng, YM |
| 刊名 | IUBMB LIFE
 |
| 出版日期 | 2001 |
| 卷号 | 51期号:1页码:33-37 |
| 关键词 | IGF-1 insulin insulin structural motif scaffold molecule |
| 通讯作者 | Feng, YM (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Three recombinant single-chain insulin mutants, [B10Glu]Ins/ IGF-1(C), [A5Glu]PIP, and [A8Tyr]PIP were prepared by means of protein engineering, In the three mutants, the residue at position 10 of insulin B chain and positions 5 and 8 of insulin A chain were substituted by the corresponding residue Glu9, Glu46, and an aromatic residue at 49 of IGF-1, respectively. The growth-promoting activity of [B18Glu]Ins/IGF-1(C) is 58-fold higher than insulin and of [A5Glu]PIP, and [A8Tyr]PIP is 2.5- and 4.7-fold higher than PIP, respectively, measured using a mouse mammary tumor cell line GR2H6 in vitro, The results show that the residues Glu9, Glu46, and Phe49, especially the Glu9, play an important role in the growth-promoting function of IGF-1, Based on the results, we suggest that the insulin could be used as a scaffold to investigate the contributions of residues of IGF-1 to its biological function. |
| 学科主题 | Biochemistry & Molecular Biology ; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | FACTOR-I ; RECEPTOR-BINDING ; RELAXIN ; HYBRID ; PEPTIDE ; CHAIN ; CDNA ; EVOLUTION ; AMPHIOXUS ; PRECURSOR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000169228900005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2590]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Chen, H,Feng, YM. Contribution of the residue Glu9, Glu46, and Phe49 to the biological activity of insulin-like growth factor-1[J]. IUBMB LIFE,2001,51(1):33-37. |
| APA | Chen, H,&Feng, YM.(2001).Contribution of the residue Glu9, Glu46, and Phe49 to the biological activity of insulin-like growth factor-1.IUBMB LIFE,51(1),33-37. |
| MLA | Chen, H,et al."Contribution of the residue Glu9, Glu46, and Phe49 to the biological activity of insulin-like growth factor-1".IUBMB LIFE 51.1(2001):33-37. |
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