B lymphocytes and plasma cells express functional E-selectin by constitutive activation of NF-kappa B
文献类型：期刊论文
| 作者 | Liu, LP; Xia, YF; Yang, L; DiDonato, JA; DiCorleto, PE; Zhong, CP; Geng, JG |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2001 |
| 卷号 | 286期号:2页码:281-291 |
| 关键词 | E-selectin adhesion molecules B-lymphocytes plasma cells NF-kappa B transcriptional regulation |
| 通讯作者 | Geng, JG (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Room 505,320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | E-selectin (CD62E), a cell adhesion molecule for most leukocytes, is known to be expressed exclusively on the cytokine-stimulated endothelial cells mainly by inductive activation of NF-kappaB. Using immunohistochemistry and in situ hybridization, we showed that B lymphocytes and plasma cells in the spleens and lymph nodes from nude mice (T-lymphocyte-deficient), but not from SCID mice (T- and B-lymphocyte-deficient), expressed E-selectin prior to cytokine stimulation. The expression of E-selectin was also confirmed on human B lymphocytes isolated from peripheral bloods. The mouse J774A.1 monocytes could adhere to the marginal zones of mouse spleens in an E-selectin Ab inhibitable manner, suggesting the functional activity of the expressed E-selectin. In addition, ARH-77 cells, a cell line derived from human plasma cells, were found to express E-selectin mRNA and protein and to have a NF-kappaB activity for an E-selectin promoter. NF-kappaB antagonists, such as TPCK (tosylsulfonyl phenylalanyl chloromethyl ketone), dexamethasone and a I kappaB alpha mutant plasmid could inhibit both the NF-kappaB activity and the expression of E-selectin. Transfection with an E-selectin promoter-driven reporter gene construct further verified the E-selectin promoter activity in ARH-77 cells. Again, TPCK, dexamethasone, and the I kappaB alpha mutant plasmid could neutralize this activity. These findings suggest that B lymphocytes and plasma cells can express E-selectin, which is functional for monocytic leukocytes, by a mechanism of constitutive activation of NF-kappaB. (C) 2001 Academic Press. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | DEPENDENT ADHESION EFFICIENCY ; COLON-CARCINOMA CELLS ; P-SELECTIN ; ENDOTHELIAL-CELLS ; CANCER CELLS ; TUMOR-CELLS ; LIVER ; FLOW ; PHOSPHORYLATION ; METASTASIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000170606200010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2593]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, LP,Xia, YF,Yang, L,et al. B lymphocytes and plasma cells express functional E-selectin by constitutive activation of NF-kappa B[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2001,286(2):281-291. |
| APA | Liu, LP.,Xia, YF.,Yang, L.,DiDonato, JA.,DiCorleto, PE.,...&Geng, JG.(2001).B lymphocytes and plasma cells express functional E-selectin by constitutive activation of NF-kappa B.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,286(2),281-291. |
| MLA | Liu, LP,et al."B lymphocytes and plasma cells express functional E-selectin by constitutive activation of NF-kappa B".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 286.2(2001):281-291. |
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