Cloning and characterization of F-LANa, upregulated in human liver cancer
文献类型：期刊论文
| 作者 | Ying, H; Yu, YL; Xu, YH |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2001 |
| 卷号 | 286期号:2页码:394-400 |
| 关键词 | differential display human F-LANa in silico cloning evolutionarily conserved gene structure analysis cell growth antisense oligonucleotide |
| 通讯作者 | Xu, YH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Lab Mol & Cellular Oncol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Differentially expressed genes between normal liver and hepatocellular carcinomas were investigated using differential display. Consequently, we identified a fragment cDNA upregulated in tumor tissues. We screened the liver library and cloned the full-length cDNA, named F-LANa. Increased expression of F-LANa was confirmed by Northern blot analysis in 10 of 14 (71%) cases of hepatocellular carcinomas. Human F-LANa gene maps to chromosome 17p at D17S1828-D17S786, spans at least 11.8 kb, and contains 7 exons. This gene encodes a 239 aa protein exhibiting 97.9% similarity to the mouse ortholog gene, identified later by in silico cloning. Homology analysis was carried out in various species and showed that F-LANa was evolutionarily conserved from yeast to human. In addition, F-LANa antisense oligonucleotide suppressed F-LANa expression in human hepatocellular carcinoma BEL-7404 cells and significantly inhibited cell growth. Together, our data demonstrate that overexpression of evolutionarily conserved F-LANa occurs frequently and may play an important role in proliferation. (C) 2001 Academic Press. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | HUMAN HEPATOCELLULAR CARCINOMAS ; GENES ; IDENTIFICATION ; GENETICS ; MODEL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000170606200027 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2594]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Ying, H,Yu, YL,Xu, YH. Cloning and characterization of F-LANa, upregulated in human liver cancer[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2001,286(2):394-400. |
| APA | Ying, H,Yu, YL,&Xu, YH.(2001).Cloning and characterization of F-LANa, upregulated in human liver cancer.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,286(2),394-400. |
| MLA | Ying, H,et al."Cloning and characterization of F-LANa, upregulated in human liver cancer".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 286.2(2001):394-400. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。