中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Characterization of the genomic structure and tissue-specific promoter of the human nuclear receptor NR5A2 (hB1F) gene

文献类型:期刊论文

作者Zhang, CK; Lin, W; Cai, YN; Xu, PL; Dong, H; Li, M; Kong, YY; Fu, G; Xie, YH; Huang, GM
刊名GENE
出版日期2001
卷号273期号:2页码:239-249
关键词nuclear receptor fushi tarazu factor 1 hepatocyte nuclear factors
通讯作者Wang, Y (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,
英文摘要The human homologue of the Drosophila melanogaster orphan nuclear receptor fushi tarazu factor 1 (Ftz-F1),NR5A2(hB1F), was initially identified as a regulatory factor that binds and activates enhancer II of hepatitis B virus. NR5A2 (hB1F) is expressed specifically in pancreas and liver, playing important roles in the regulation of several liver-specific genes. A detailed analysis on the genomic structure and promoter activity will greatly promote future studies on the function of the NR5A2 (hB1F) gene. In this report, a bacterial artificial chromosome clone and several phage clones covering the NR5A2 (hB1F) Gene were isolated and the complete genomic sequence was obtained. Alignment of different cDNAs of the NR5A2 (hB1F) gene with the genomic sequence facilitated the delineation of its structural organization, which spans over 150 kb and consists of ei-ht exons interrupted by seven introns. RT-PCR and 3 ' -RACE revealed that utilization of two polyadenylation signals results in the 3.8 and 5.2 kb transcripts that were observed previously. The transcription start site of the NR5A2 (hB1F) gene was mapped downstream of a canonical TATA box. An upstream fragment containing binding sites for several liver-specific and ubiquitous transcription factors exhibits hepatocyte-specific promoter activity. Transient transfections indicated that hepatocyte nuclear factors HNF1 and HNF3 beta could activate NR5A2 (hB1F) promoter. (C) 2001 Elsevier Science B.V. All rights reserved.
学科主题Genetics & Heredity
类目[WOS]Genetics & Heredity
关键词[WOS]HOMEODOMAIN PROTEIN FTZ ; TRANSCRIPTION FACTOR ; FORK-HEAD ; SUPERFAMILY ; ACTIVATION ; COFACTOR ; SEQUENCE ; SEARCH ; FAMILY
收录类别SCI
语种英语
WOS记录号WOS:000171151100010
版本出版稿
源URL[http://202.127.25.143/handle/331003/2595]  
专题上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式
GB/T 7714
Zhang, CK,Lin, W,Cai, YN,et al. Characterization of the genomic structure and tissue-specific promoter of the human nuclear receptor NR5A2 (hB1F) gene[J]. GENE,2001,273(2):239-249.
APA Zhang, CK.,Lin, W.,Cai, YN.,Xu, PL.,Dong, H.,...&Wang, Y.(2001).Characterization of the genomic structure and tissue-specific promoter of the human nuclear receptor NR5A2 (hB1F) gene.GENE,273(2),239-249.
MLA Zhang, CK,et al."Characterization of the genomic structure and tissue-specific promoter of the human nuclear receptor NR5A2 (hB1F) gene".GENE 273.2(2001):239-249.

入库方式: OAI收割

来源:上海生物化学与细胞生物学研究所

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