Bcl-2 over-expression and activation of protein kinase C suppress the Trail-induced apoptosis in Jurkat T cells
文献类型：期刊论文
| 作者 | Guo, BC; Xu, YH |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2001 |
| 卷号 | 11期号:2页码:101-106 |
| 关键词 | Trail Jurkat T cells apoptosis Bcl-2 PMA PKC bis |
| 通讯作者 | Xu, YH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Trail, a tumor necrosis factor-related apoptosis-inducing ligand, is a novel potent endogenous activator of the cell death pathway through the activation of cell surface death receptors Trail-Ri and Trail-Ra. Its role, like Fast in activation-induced cell death (AICD), has been demonstrated in immune system. However the mechanism of Trail induced apoptosis remains unclear. In this report, the recombinant Trail protein was expressed and purified. The apoptosis-inducing activity and the regulation mechanism of recombinant Trail on Jurkat T cells were explored in vitro. Trypan blue exclusion assay demonstrated that the recombinant Trail protein actively killed Jurkat T cells in a dose-dependent manner. Trail-induced apoptosis in Jurkat T cells were remarkably reduced by Bcl-2 over expression in Bcl-2 gene transfected cells. Treatment with PMA (phorbol 12-myristate 13-acetate), a PKC activator, suppressed Trail-induced apoptosis in Jurkat T cells. The inhibition of apoptosis by PMA was abolished by pretreatment with His, a PKC inhibitor. Taken together, it was suggested that Bcl-2 over-expression and PMA activated PKC actively down-regulated the Trail-mediated apoptosis in Jurkat T cell. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | APO2 LIGAND ; DEATH ; MITOCHONDRIA ; INVOLVEMENT ; CASPASE-8 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000169787000004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2598]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Guo, BC,Xu, YH. Bcl-2 over-expression and activation of protein kinase C suppress the Trail-induced apoptosis in Jurkat T cells[J]. CELL RESEARCH,2001,11(2):101-106. |
| APA | Guo, BC,&Xu, YH.(2001).Bcl-2 over-expression and activation of protein kinase C suppress the Trail-induced apoptosis in Jurkat T cells.CELL RESEARCH,11(2),101-106. |
| MLA | Guo, BC,et al."Bcl-2 over-expression and activation of protein kinase C suppress the Trail-induced apoptosis in Jurkat T cells".CELL RESEARCH 11.2(2001):101-106. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。