Adenovirus-mediated expression of pig alpha(1,3) galactosyltransferase reconstructs Gal alpha(1,3) Gal epitope on the surface of human tumor cells
文献类型：期刊论文
| 作者 | Xing, L; Xia, GH; Fei, J; Huang, F; Guo, LH |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2001 |
| 卷号 | 11期号:2页码:116-124 |
| 关键词 | adenoviral vector galactosyltransferase Gal alpha(1 gene expression human tumor cell 3) Gal |
| 通讯作者 | Guo, LH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | Gal alpha (1, 3) Gal (gal epitope) is a carbohydrate epitope and synthesized in large amount by ol(l, 3) galactosyltransferase [alpha (1, 3) GT] enzyme on the cells of lower mammalian animals such as pigs and mice. Human has no gal epitope due to the inactivation of alpha (1, 3) GT gene but produces a large amount of antibodies (anti-Gal) which recognize Gal alpha (I, 3) Gal structures specifically. In this study, a replication-deficient recombinant adenoviral vector Ad5sGT containing pig alpha (1, 3) GT cDNA was constructed and characterized. Adenoviral vector-mediated transfer of pig alpha (l, 3) GT gene into human tumor cells such as malignant melanoma A375, stomach cancer SGC-7901, and lung cancer SPC-A-1 was reported for the first time. Results showed that Gal epitope did not increase the sensitivity of human tumor cells to human complement-mediated lysis, although human complement activation and the binding of human IgG and IgM natural antibodies to human tumor cells were enhanced significantly after Ad5sGT transduction. Appearance of gal epitope on the human tumor cells changed the expression of cell surface carbohydrates reacting with Ulex europaeus I (UEA I) lectins, Vicia villosa agglutinin (VVA), Arachis hypogaea agglutinin (PNA), and Glycine max agglutinin (SBA) to different degrees. In addition, no effect of gal epitope on the growth in vitro of human tumor cells was observed in MTT assay. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | BLOOD-GROUP ANTIGEN ; GALACTOSYL EPITOPES ; TRANSGENIC MICE ; GENE-THERAPY ; VACCINES ; CANCER ; ALPHA-1,3GALACTOSYLTRANSFERASE ; ALPHA-1,2-FUCOSYL-TRANSFERASE ; GAL-ALPHA(1,3)GAL ; IMMUNOGENICITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000169787000006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2599]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xing, L,Xia, GH,Fei, J,et al. Adenovirus-mediated expression of pig alpha(1,3) galactosyltransferase reconstructs Gal alpha(1,3) Gal epitope on the surface of human tumor cells[J]. CELL RESEARCH,2001,11(2):116-124. |
| APA | Xing, L,Xia, GH,Fei, J,Huang, F,&Guo, LH.(2001).Adenovirus-mediated expression of pig alpha(1,3) galactosyltransferase reconstructs Gal alpha(1,3) Gal epitope on the surface of human tumor cells.CELL RESEARCH,11(2),116-124. |
| MLA | Xing, L,et al."Adenovirus-mediated expression of pig alpha(1,3) galactosyltransferase reconstructs Gal alpha(1,3) Gal epitope on the surface of human tumor cells".CELL RESEARCH 11.2(2001):116-124. |
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