Maternal zinc deficiency impairs brain nestin expression in prenatal and postnatal mice
文献类型：期刊论文
| 作者 | Wang, FD; Bian, W; Kong, LW; Zhao, FJ; Guo, JS; Jing, NH |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2001 |
| 卷号 | 11期号:2页码:135-141 |
| 关键词 | zinc deficiency nestin brain development neural stem cells |
| 通讯作者 | Jing, NH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Effects of maternal dietary zinc deficiency on prenatal and postnatal brain development were investigated in ICR strain mice. From d 1 of pregnancy (EO) until postnatal d 20 (P20), maternal mice were fed experimental diets that contained 1 mg Zn/kg/day (severe zinc deficient, SZD), 5 mg Zn/kg/day (marginal zinc deficient, MZD), 30 mg Zn/kg/day (zinc adequately supplied, ZA) or 100 mg Zn/kg/day (zinc supplemented, ZS and pair-fed, PF). Brains of offspring from these dietary groups were examined at various developmental stages for expression of nestin, an intermediate filament protein found in neural stem cells and young neurons. Immunocytochemistry showed nestin expression in neural tube 10.5 d post citrus (dpc) as well as in the cerebral cortex and neural tube from 10.5 dpc to postnatal d 10 (P10). Nestin immunoreactivities in both brain and neural tube of those zinc-supplemented control groups (ZA, ZS, PF) were stronger than those in zinc-deficient groups (SZD and MZD). Western blot analysis confirmed that nestin levels in pooled brain extracts from each of the zinc-supplemented groups (ZA, ZS, PF) were much higher than those from the zinc-deficient groups (SZD and MZD) from 10.5 dpc to P10. Immunostaining and Western blots showed no detectable nestin in any of the experimental and control group brains after P20. These observations of an association between maternal zinc deficiency and decreased nestin protein levels in brains of offspring suggest that zinc deficiency suppresses development of neural stem cells, an effect which may lead to neuroanatomical and behavioral abnormalities in adults. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | CENTRAL-NERVOUS-SYSTEM ; PRECURSOR CELLS ; TRACE-ELEMENTS ; STEM-CELLS ; RATS ; PROTEIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000169787000008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2600]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, FD,Bian, W,Kong, LW,et al. Maternal zinc deficiency impairs brain nestin expression in prenatal and postnatal mice[J]. CELL RESEARCH,2001,11(2):135-141. |
| APA | Wang, FD,Bian, W,Kong, LW,Zhao, FJ,Guo, JS,&Jing, NH.(2001).Maternal zinc deficiency impairs brain nestin expression in prenatal and postnatal mice.CELL RESEARCH,11(2),135-141. |
| MLA | Wang, FD,et al."Maternal zinc deficiency impairs brain nestin expression in prenatal and postnatal mice".CELL RESEARCH 11.2(2001):135-141. |
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