Effect of membrane fluidity on tyrosine kinase activity of reconstituted epidermal growth factor receptor
文献类型：期刊论文
| 作者 | Ge, GX; Wu, J; Lin, QS |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2001 |
| 卷号 | 282期号:2页码:511-514 |
| 关键词 | epidermal growth factor (EGF) EGF receptor tyrosine kinase reconstitution liposome membrane fluidity alamethicin |
| 通讯作者 | Lin, QS (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Epidermal growth factor receptor (EGFR) was functionally reconstituted into liposome membrane. Triton X-100 was removed by Bio-beads SM-2, More than 80% of the reconstituted EGFR possessed right-side-out orientation with the EGF binding side facing the medium. The tyrosine kinase assay of the EG;FR was carried out in the presence of the antibiotic alamethicin, The reconstituted EGFR tyrosine kinase was well activated by EGF. The influence of lipid composition on tyrosine kinase activity was investigated, Introduction of cholesterol into the dioleoylphophatidylcholine (DOPC) liposome membrane resulted in the decrease of tyrosine kinase activity. The tyrosine kinase activity of EGFR in distearylphosphatidylcholine liposome was much lower than that of EGFR-DOPC proteoliposome, Results indicated the importance of membrane fluidity on the apparent tyrosine kinase activity of reconstituted EGFR. (C) 2001 Academic Press. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | EGF-RECEPTOR ; ACTIVATION ; PHOSPHOINOSITIDES ; DIMERIZATION ; SURFACE ; BINDING ; ATPASE ; CELLS ; MODEL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000167798500023 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2606]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Ge, GX,Wu, J,Lin, QS. Effect of membrane fluidity on tyrosine kinase activity of reconstituted epidermal growth factor receptor[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2001,282(2):511-514. |
| APA | Ge, GX,Wu, J,&Lin, QS.(2001).Effect of membrane fluidity on tyrosine kinase activity of reconstituted epidermal growth factor receptor.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,282(2),511-514. |
| MLA | Ge, GX,et al."Effect of membrane fluidity on tyrosine kinase activity of reconstituted epidermal growth factor receptor".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 282.2(2001):511-514. |
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