Organization of human ACAT-2 gene and its cell-type-specific promoter activity
文献类型：期刊论文
| 作者 | Song, BL; Qi, W; Yang, XY; Chang, CCY; Zhu, JQ; Chang, TY; Li, BL |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2001 |
| 卷号 | 282期号:2页码:580-588 |
| 关键词 | ACAT-2 genomic organization promoter IGFBP-6 |
| 通讯作者 | Chang, TY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Acyl-CoA:cholesterol acyltransferase (ACAT) plays important roles in cellular cholesterol homeostasis. Two ACAT genes exist in mammals. We report here the genomic organization of human ACAT-2 gene and analysis of its promoter activity in various cell lines. The human ACAT-2 gene spans over 18 kb and contains 15 exons, Three transcription start sites and one poly(A) site are identified by the 5'/3'-RACE. In addition, the human ACAT-2 gene is linked to the insulinlike growth factor binding protein 6 (IGFBP-6) gene in a head-to tail manner with a small intergenic region of about 1.2 kb. The 5'-flanking region of human ACAT-2 gene contains many potential cis-acting elements for multiple transcriptional regulatory factors but lacks TATA and CCAAT boxes. Using promoter-luciferase reporter assays, we demonstrate the transcriptional activity of ACAT-2 gene promoter is high in Caco-2 cells, especially after these cells become postconfluent and behave as intestinal enterocytes. (C) 2001 Academic Press. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | ACYL-COENZYME-A ; CHOLESTEROL ACYLTRANSFERASE-1 ACAT-1 ; HUMAN HEPATOCELLULAR-CARCINOMA ; CALBINDIN-D9K GENE ; SMALL-INTESTINE ; MESSENGER-RNA ; HUMAN LIVER ; EXPRESSION ; TRANSCRIPTION ; MACROPHAGES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000167798500034 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2607]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Song, BL,Qi, W,Yang, XY,et al. Organization of human ACAT-2 gene and its cell-type-specific promoter activity[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2001,282(2):580-588. |
| APA | Song, BL.,Qi, W.,Yang, XY.,Chang, CCY.,Zhu, JQ.,...&Li, BL.(2001).Organization of human ACAT-2 gene and its cell-type-specific promoter activity.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,282(2),580-588. |
| MLA | Song, BL,et al."Organization of human ACAT-2 gene and its cell-type-specific promoter activity".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 282.2(2001):580-588. |
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