Identification of up-regulated genes in rat regenerating liver tissue by suppression subtractive hybridization
文献类型：期刊论文
| 作者 | Liu, ZW; Zhao, MJ; Li, ZP |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2001 |
| 卷号 | 33期号:2页码:191-197 |
| 关键词 | gene screening liver regeneration suppression subtractive hybridization |
| 通讯作者 | , |
| 英文摘要 | Suppression subtractive hybridization (SSH) technique was used to screen up-regulated genes in regenerating liver, The cDNA from rat regenerating liver tissue was used as the tester, and that from normal liver was used as the driver. After cloning, a subtraction cDNA library of 900 clones was obtained. These 900 clones were further analyzed by differential gene expression screening, and 50 clones with obvious overexpression in regenerating liver were identified. Sequencing analysis and homology searching showed that these clones represent 37 genes, 13 of them are homologous to genes known to be involved in liver regeneration; 15 are known genes which are first found to be up-regulated in regenerating liver; and 9 are novel genes (ESTs) which have been deposited to GenBank. RNA dot blotting analysis was used to further study the gene expression patterns. The results not only confirmed the up-regulation of these genes in regenerating liver tissue, but also showed that these genes had different expression patterns during the process of liver regeneration. The results implied that these genes might play important roles in liver regeneration. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | HEPATOCYTE PROLIFERATION ; PARTIAL-HEPATECTOMY ; MESSENGER-RNA ; EXPRESSION ; MICE |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000167681800008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2609]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, ZW,Zhao, MJ,Li, ZP. Identification of up-regulated genes in rat regenerating liver tissue by suppression subtractive hybridization[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2001,33(2):191-197. |
| APA | Liu, ZW,Zhao, MJ,&Li, ZP.(2001).Identification of up-regulated genes in rat regenerating liver tissue by suppression subtractive hybridization.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,33(2),191-197. |
| MLA | Liu, ZW,et al."Identification of up-regulated genes in rat regenerating liver tissue by suppression subtractive hybridization".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 33.2(2001):191-197. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。