Screening of genes related to the expression of human epsilon-globin gene by using yeast one-hybrid system
文献类型：期刊论文
| 作者 | Huang, J; Hou, CH; Qian, RL |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2001 |
| 卷号 | 33期号:2页码:246-250 |
| 关键词 | human epsilon-globin gene yeast one-hybrid system silencer RPL3 |
| 通讯作者 | , |
| 英文摘要 | The developmental stage-specific silencing of the human E-globin gene during embryonic period is controlled, in part, by a silencer (from -392 to -177 bp) upstream of this gene. In our previous work, by using DNase I footprinting assay, a major protected region from -278 to -235 bp within this silencer DNA was identified. In order to isolate genes encoding proteins that bind to this target element, a human fetal liver cDNA library was screened by using yeast one-hybrid system. The candidate clones were sequenced, and the ribosomal protein L3 (RPL3) was preliminarily isolated. Electrophoretic mobility shift assay (EMSA) and competition tests also demonstrated its binding activity to the target: element. These imply that RPL3 might play an important role in silencing the human embryonic epsilon -globin gene expression through the interaction with this silencer. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | RIBOSOMAL-PROTEIN S1 ; TRANSCRIPTION ; CLONING ; SILENCER |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000167681800019 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2613]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Huang, J,Hou, CH,Qian, RL. Screening of genes related to the expression of human epsilon-globin gene by using yeast one-hybrid system[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2001,33(2):246-250. |
| APA | Huang, J,Hou, CH,&Qian, RL.(2001).Screening of genes related to the expression of human epsilon-globin gene by using yeast one-hybrid system.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,33(2),246-250. |
| MLA | Huang, J,et al."Screening of genes related to the expression of human epsilon-globin gene by using yeast one-hybrid system".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 33.2(2001):246-250. |
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