Characterization of glycoprotein ligands for P-selectin on a human small cell lung cancer cell line NCI-H345
文献类型：期刊论文
| 作者 | Li, L; Short, HJ; Qian, KX; Elhammer, AP; Geng, JG |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2001 |
| 卷号 | 288期号:3页码:637-644 |
| 关键词 | cell adhesion molecules P-selectin cancer metastasis |
| 通讯作者 | Geng, JG (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Room 505,320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | P-selectin (CD62P) is a cell adhesion molecule expressed on stimulated endothelial cells and on activated platelets. It interacts with PSGL-1 (P-selectin glycoprotein ligand-1; CD162) on leukocytes and mediates recruitment of leukocytes during inflammation. P-selectin also binds to several types of cancer cells in vitro and facilitates growth and metastasis of colon carcinoma in vivo. Here we show that P-selectin, but not E-selectin, binds to NCI-H345 cells, a cell line derived from a human small cell lung cancer. EDTA or P7 (a leukocyte adhesion blocking mAb to P-selectin), but not PL5 (a leukocyte adhesion blocking mAb to PSGL-1), can inhibit this binding. P-selectin affinity chromatography can precipitate a similar to 110-kDa major band and a similar to 220-kDa minor band from [H-3]-glucosamine-labeled NCI-H345 cells. No expression of PSGL-1 protein and m-RNA can be detected in NCI-H345 cells. Taken together, these results suggest that NCI-H345 cells express glycoprotein ligands for P-selectin that are distinct from leukocyte PSGL-1. (C) 2001 Academic Press. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | ADHESION MOLECULES ; MEDIATES ADHESION ; AMINO-TERMINUS ; PSGL-1 ; IDENTIFICATION ; RECOGNITION ; NEUTROPHILS ; EXPRESSION ; MONOCYTES ; MEMBRANE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000172077500022 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2616]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, L,Short, HJ,Qian, KX,et al. Characterization of glycoprotein ligands for P-selectin on a human small cell lung cancer cell line NCI-H345[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2001,288(3):637-644. |
| APA | Li, L,Short, HJ,Qian, KX,Elhammer, AP,&Geng, JG.(2001).Characterization of glycoprotein ligands for P-selectin on a human small cell lung cancer cell line NCI-H345.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,288(3),637-644. |
| MLA | Li, L,et al."Characterization of glycoprotein ligands for P-selectin on a human small cell lung cancer cell line NCI-H345".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 288.3(2001):637-644. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。