Non-specific deadenylation and deguanylation of naked RNA catalyzed by ricin under acidic condition
文献类型：期刊论文
| 作者 | Tang, S; Xie, L; Hou, FJ; Liu, WY; Ruan, KC |
| 刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
 |
| 出版日期 | 2001 |
| 卷号 | 1519期号:3页码:192-198 |
| 关键词 | deguanylation ribosome-inactivating protein ricin RNA N-glycosidase sarcin/ricin domain RNA |
| 通讯作者 | Liu, WY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Ricin A-chain catalyzes the hydrolysis of the N-glycosidic bond of a conserved adenosine residue at position 4324 in the sarcin/ricin domain of 28S RNA of rat ribosome. The GAGA tetraloop closed by C-G pairs is required for recognition of the cleavage site on 28S ribosomal RNA by ricin A-chain. In this study, ricin A-chain (reduced ricin) exhibits specific depurination on a synthetic oligoribonucleotide (named SRD RNA) mimic of the sarcin/ricin domain of rat 28S ribosomal RNA under neutral and weak acidic conditions. Furthermore, the activity of intact ricin is also similar to that of ricin A-chain. However, under more acidic conditions, both enzymes lose their site specificity. The alteration in specificity of depurination is not dependent on the GAGA tetraloop of SRD RNA. A higher concentration of KCI inhibits the non-specific N-glycosidase activity much more than the specific activity of ricin A-chain. In addition, characterization of depurination sites by RNA sequencing reveals that under acidic conditions ricin A-chain can release not only adenines, but also guanines from SRD RNA or 5S ribosomal RNA. This is the first report of the non-specific deadenylation and deguanylation activity of ricin A-chain to the naked RNA under acidic conditions. (C) 2001 Elsevier Science B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | RIBOSOME-INACTIVATING PROTEINS ; A-CHAIN ; SUBSTRATE-ANALOGS ; ALPHA-SARCIN ; ACTIVE-SITE ; TOXIN ; MECHANISM ; DYNAMICS ; ENTRY ; HIV-1 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000169589600005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2621]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Tang, S,Xie, L,Hou, FJ,et al. Non-specific deadenylation and deguanylation of naked RNA catalyzed by ricin under acidic condition[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION,2001,1519(3):192-198. |
| APA | Tang, S,Xie, L,Hou, FJ,Liu, WY,&Ruan, KC.(2001).Non-specific deadenylation and deguanylation of naked RNA catalyzed by ricin under acidic condition.BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION,1519(3),192-198. |
| MLA | Tang, S,et al."Non-specific deadenylation and deguanylation of naked RNA catalyzed by ricin under acidic condition".BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION 1519.3(2001):192-198. |
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